NF-L Polyklonaler Antikörper

NF-L Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 12998-1-AP

Synonyme

NEFL, NFL, NF68, neurofilament

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Galerie der Validierungsdaten

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  WB analysis of mouse brain tissue using 12998-1-AP

  WB result of 12998-1-AP.

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  WB analysis using 12998-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 12998-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12998-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12998-1-AP (NF-L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12998-1-AP

  IP result of anti-NF-L (IP:12998-1-AP, 4ug; Detection:12998-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12998-1-AP (NF-L antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 12998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 12998-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SH-SY5Y using 12998-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using NF-L antibody (12998-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of SH-SY5Y using 12998-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human NF-L (12998-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, C6-Zellen, Maushirngewebe, Neuro-2a-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen
• Erfolgreiche Detektion in FC (Intra): SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

12998-1-AP bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA NF-L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NF-L fusion protein Ag3667
• Vollständiger Name: neurofilament, light polypeptide
• Berechnetes Molekulargewicht: 543 aa, 62 kDa
• Beobachtetes Molekulargewicht: 65-68 kDa, 140-160 kDa
• GenBank-Zugangsnummer: BC039237
• Gene symbol: NF-L
• Gene ID (NCBI): 4747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.; Authors - Zhengxin Ying
mouse	WB	Mater Today Bio; Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.; Authors - Luyu Wang
mouse	WB	Mater Today Bio; In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.; Authors - Dan Zhang
rat	WB,IF	Adv Sci (Weinh); An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.; Authors - Jing Liu
		Front Mol Neurosci; Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma.; Authors - Mumtaz Kasim
mouse	WB	Hum Mol Genet; Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.; Authors - Peng-Peng Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Max (Verified Customer) (02-24-2022)

strong and specific labeling of NfL in hamster brain lysate on western blot

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hamster brain lysate

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