NF-H/NF200 Polyclonal antibody
NF-H/NF200 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 21471-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 3 publications below |
Product Information
The immunogen of 21471-1-AP is NF-H/NF200 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NF-H/NF200 fusion protein Ag15249 |
| Full Name | neurofilament, heavy polypeptide |
| Calculated Molecular Weight | 1026 aa, 112 kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | BC073969 |
| Gene Symbol | NF-H |
| Gene ID (NCBI) | 4744 |
| RRID | AB_10734324 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68kd; NF-M,145-160kd and NF-H, 200-220kd. This antibody recognize NEFH only.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NF-H/NF200 antibody 21471-1-AP | Download protocol |
| IHC protocol for NF-H/NF200 antibody 21471-1-AP | Download protocol |
| FC protocol for NF-H/NF200 antibody 21471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mater Today Bio Ropivacaine microsphere-loaded electroconductive nerve dressings for long-acting analgesia and functional recovery following diabetic peripheral nerve injury | ||
Sci Total Environ Neurotoxicity and biomarkers of zinc oxide nanoparticles in main functional brain regions and dopaminergic neurons. | ||
iScience Synthetic amyloid-β oligomers drive early pathological progression of Alzheimer's disease in nonhuman primates. | ||
Toxicol Appl Pharmacol Rosmarinic acid exerts a neuroprotective effect on spinal cord injury by suppressing oxidative stress and inflammation via modulating the Nrf2/HO-1 and TLR4/NF-κB pathways. | ||
Plast Reconstr Surg Glob Open A Novel, Sterilized Microvascular Tissue Product Improves Healing in a Murine Pressure Ulcer Model. |
