NDUFB6 Polyklonaler Antikörper

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• KD/KO Validated

NDUFB6 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 16037-1-AP

Synonyme

B17, CI, CI B17, Complex I B17, NDUFB6

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Galerie der Validierungsdaten

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  WB analysis using 16037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16037-1-AP (NDUFB6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16037-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16037-1-AP (NDUFB6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16037-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16037-1-AP (NDUFB6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, LNCaP-Zellen, Maus-Skelettmuskelgewebe, MCF-7-Zellen, Ratten-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

16037-1-AP bindet in WB, IHC, ELISA NDUFB6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NDUFB6 fusion protein Ag8908
• Vollständiger Name: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 6, 17kDa
• Berechnetes Molekulargewicht: 128 aa, 15 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC009801
• Gene symbol: NDUFB6
• Gene ID (NCBI): 4712
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NDUFB6, also named as NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 or Complex I-B17, is a 128 amino acid protein, which belongs to the complex I NDUFB6 subunit family. NDUFB6 localizes in the Mitochondrion inner membrane and is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Publikationen

Species	Application	Title
mouse	WB, IHC	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang
human,mouse	WB	EMBO Rep; OMA1 reprograms metabolism under hypoxia to promote colorectal cancer development.; Authors - Zhida Wu ; KD Validated
	WB	Redox Biol; Mitochondrial complex I deactivation is related to superoxide production in acute hypoxia.; Authors - Pablo Hernansanz-Agustín
mouse	WB	J Nutr Biochem; Deficiency of β-carotene oxygenase 2 induces mitochondrial fragmentation and activates the STING-IRF3 pathway in the mouse hypothalamus.; Authors - Lei Wu
human	WB	Mitochondrion; MNRR1 (formerly CHCHD2) is a bi-organellar regulator of mitochondrial metabolism.; Authors - Siddhesh Aras
mouse	WB	FEBS Lett; Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation.; Authors - Shen Shao-Ming SM

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