NCKAP1 Polyklonaler Antikörper

NCKAP1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 12140-1-AP

Synonyme

NAP125, NAP1, NAP 1, Membrane-associated protein HEM-2, KIAA0587

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Galerie der Validierungsdaten

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  WB analysis using 12140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12140-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12140-1-AP (NCKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 12140-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12140-1-AP (NAP1;NCKAP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of mouse brain using 12140-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NCKAP1 antibody (12140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, rat brain
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below

Produktinformation

12140-1-AP bindet in WB, IHC, IF-P, ELISA NCKAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NCKAP1 fusion protein Ag2788
• Vollständiger Name: NCK-associated protein 1
• Berechnetes Molekulargewicht: 129 kDa
• Beobachtetes Molekulargewicht: 115-125 kDa
• GenBank-Zugangsnummer: BC015025
• Gene symbol: NCKAP1
• Gene ID (NCBI): 10787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NCK-associated protein 1 (NCKAP1), also known as NAP1 or HEM2, is encoded by an apoptosis-related gene human NAP1, the expression of which is strongly down-regulated in sporadic Alzheimer's disease (AD). Human NAP1 is predominantly expressed in neuronal cells. Antisense oligo DNA of human NAP1 transcripts was found to induce apoptosis of neuronal cells. Loss of NAP1 function disrupts neuronal differentiation. Likewise, NAP1 plays an essential role in facilitating neuronal cytoskeletal changes underlying the postmigratory differentiation of cortical neurons, a critical step in functional wiring of the cortex. Human NAP1 is also proved to be an orthologue of rat Nap1 which binds to the adaptor molecule NCK in signal transduction.

Publikationen

Species	Application	Title
human	WB	Cell Res; Nudel is crucial for the WAVE complex assembly in vivo by selectively promoting subcomplex stability and formation through direct interactions.; Authors - Wu Shuang S
human,mouse	WB	Curr Biol; Parallel kinase pathways stimulate actin polymerization at depolarized mitochondria.; Authors - Tak Shun Fung
mouse	WB	FASEB J; Tropomyosin-related kinase C (TrkC) enhances podocyte migration by ERK-mediated WAVE2 activation.; Authors - Sascha Gromnitza
		Biochem J; Selenoprotein S is Involved in Maintenance and Transport of Multiprotein Complexes.; Authors - Anton A Turanov
mouse	WB	Sci Rep; Synaptic GAP and GEF Complexes Cluster Proteins Essential for GTP Signaling.; Authors - Brent Wilkinson
human	WB	World J Gastroenterol; Abelson interactor 1 splice isoform-L plays an anti-oncogenic role in colorectal carcinoma through interactions with WAVE2 and full-length Abelson interactor 1.; Authors - Kun Li

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