MYOD1 Polyklonaler Antikörper

MYOD1 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (7)

Anwendung

WB, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18943-1-AP

Synonyme

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe, humanes Herzgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
Erfolgreiche Detektion in IHCMausembryogewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FCC2C12-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
Immunhistochemie (IHC)IHC : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18943-1-AP bindet in WB, IHC, IF, FC, ChIP, ELISA MYOD1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Ente, Hausschwein, Huhn, Kaninchen, Maus, Ratte, Rind, Zebrafisch, Ziege
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MYOD1 fusion protein Ag13512
Vollständiger Name myogenic differentiation 1
Berechnetes Molekulargewicht 320 aa, 35 kDa
Beobachtetes Molekulargewicht 35-45 kDa
GenBank-ZugangsnummerBC064493
Gene symbol MYOD1
Gene ID (NCBI) 4654
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

Protokolle

Produktspezifische Protokolle
WB protocol for MYOD1 antibody 18943-1-APProtokoll herunterladen
IHC protocol for MYOD1 antibody 18943-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
mouseWB

Nat Commun

The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.

Authors - Giovanna Rigillo
mouseWB

J Cachexia Sarcopenia Muscle

circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.

Authors - Junyu Yan
humanWB,IHC

Cancer Res

YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.

Authors - Shuai Ye

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Cassandra (Verified Customer) (02-04-2021)

This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Satellite cell
MYOD1 Antibody Immunofluorescence validation (1:100 dilution) in Satellite cell (Cat no:18943-1-AP)