MYL7 Polyklonaler Antikörper

MYL7 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Rezensionen (2)

Kat-Nr. : 17283-1-AP

Synonyme

MLC 2a, MLC2a, MYL2A, MYL7, MYLC2A, Myosin light chain 2a

---

Galerie der Validierungsdaten

• 

  WB analysis using 17283-1-AP

  Mouse heart lysates were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17283-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 17283-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17283-1-AP (MYL7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 17283-1-AP

  IP Result of anti-MYL7 (IP:17283-1-AP, 4ug; Detection:17283-1-AP 1:700) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of mouse heart using 17283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17283-1-AP (MYL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17283-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17283-1-AP (MYL7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 17283-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 17283-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYL7 antibody (17283-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of C2C12 using 17283-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.5 ug Anti-Human MYL7 (17283-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe
• Erfolgreiche Detektion in FC: C2C12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below

Produktinformation

17283-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA MYL7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MYL7 fusion protein Ag11058
• Vollständiger Name: myosin, light chain 7, regulatory
• Berechnetes Molekulargewicht: 175 aa, 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC027915
• Gene symbol: MYL7
• Gene ID (NCBI): 58498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MYL7, also known as myosin light chain 2a (MLC2a), is essential for heart development. The expression of MYL7 in heart is predominatantly restricted to the atrium. So its expression has been considered as useful marker for atrial cardiomyocytes.

Publikationen

Species	Application	Title
	IF	J Clin Invest; 1-Deoxynojirimycin promotes cardiac function and rescues mitochondrial cristae in mitochondrial hypertrophic cardiomyopathy; Authors - Qianqian Zhuang
mouse	IF	Nat Commun; High-efficiency reprogramming of fibroblasts into cardiomyocytes requires suppression of pro-fibrotic signalling.; Authors - Yuanbiao Zhao
human	IF	Theranostics; Thymosin β4 increases cardiac cell proliferation, cell engraftment, and the reparative potency of human induced-pluripotent stem cell-derived cardiomyocytes in a porcine model of acute myocardial infarction.; Authors - Shi Hua Tan
human	IF	Cardiovasc Res; Angiopoietin-1 enhanced myocyte mitosis, engraftment, and the reparability of hiPSC-CMs for treatment of myocardial infarction.; Authors - Zhonghao Tao
human	WB	Stem Cell Reports; Mitochondrial Dysfunctions Contribute to Hypertrophic Cardiomyopathy in Patient iPSC-Derived Cardiomyocytes with MT-RNR2 Mutation.; Authors - Shishi Li
human	IF	Front Cell Dev Biol; HiPS-Endothelial Cells Acquire Cardiac Endothelial Phenotype in Co-culture With hiPS-Cardiomyocytes.; Authors - Emmi Helle

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Brice-Emmanuel (Verified Customer) (12-18-2023)

Works very well in WB

• Applications: Western Blot
• Primary Antibody Dilution: 1/5000
• Cell Tissue Type: Mouse Heart Tissue

MYL7 Antibody Western Blot validation (1/5000 dilution) in Mouse Heart Tissue (Cat no:17283-1-AP)

FH

Rebecca (Verified Customer) (10-25-2022)

20ug of protein were loaded. Transference was performed at 4ºC and 90V for 2h. Antibody incubation was done ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: iPSC-CM

MYL7 Antibody Western Blot validation (1:1000 dilution) in iPSC-CM (Cat no:17283-1-AP)

Verwandte Produkte

Myosin Light Chain 2/MLC-2V Polyclonal antibody

10906-1-AP

Myosin Light Chain 2/MLC-2V Monoclonal antibody

60229-1-Ig

MYH7-specific Polyclonal antibody

22280-1-AP

MYH6 Polyclonal antibody

22281-1-AP