MYH7-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

MYH7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

68

Cat no : 22280-1-AP

Synonyms

Myosin 7, MYH7, Myosin heavy chain 7, MyHC-slow, MyHC-beta

---

Validation Data Gallery

• 

  WB analysis using 22280-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22280-1-AP (MYH7-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 22280-1-AP

  IP result of anti-MYH7-specific (IP:22280-1-AP, 5ug; Detection:22280-1-AP 1:300) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of mouse heart using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22280-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 22280-1-AP (MYH7-specific antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse heart using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 22280-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using MYH7-specific antibody (22280-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 22280-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using MYH7-specific antibody (22280-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: H9C2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 58 publications below
• IHC: See 7 publications below
• IF: See 6 publications below

Product Information

22280-1-AP targets MYH7-specific in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, zebrafish, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: myosin, heavy chain 7, cardiac muscle, beta
• Calculated Molecular Weight: 223 kDa
• Observed Molecular Weight: 200-220 kDa
• GenBank Accession Number: NM_000257
• Gene Symbol: MYH7
• Gene ID (NCBI): 4625
• RRID: AB_2736821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs. (2523412, 14520662)

Publications

Species	Application	Title
human	WB	Science; HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.; Authors - John T Hinson
mouse	WB	Circulation; Hyperglycemia-Driven Inhibition of AMP-Activated Protein Kinase α2 Induces Diabetic Cardiomyopathy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes In Vivo.; Authors - Shengnan Wu
		Dev Cell; Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.; Authors - Anant Chopra
human	WB	Elife; Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp; Authors - Abigail C Neininger-Castro ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Dectin-1 deficiency alleviates diabetic cardiomyopathy by attenuating macrophage-mediated inflammatory response; Authors - Na Yang
pig	WB	J Agric Food Chem; Dietary Taurine Supplementation Improves the Meat Quality, Muscle Fiber Type, and Mitochondrial Function of Finishing Pigs; Authors - Wenting An

Related Products

Myosin Light Chain 2/MLC-2V Polyclonal antibody

10906-1-AP

ACTC1-specific Monoclonal antibody

66125-1-Ig

MYH6 Polyclonal antibody

22281-1-AP