Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22280-1-AP targets MYH7-specific in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, zebrafish, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myosin, heavy chain 7, cardiac muscle, beta |
| Calculated Molecular Weight | 223 kDa |
| Observed Molecular Weight | 200-220 kDa |
| GenBank Accession Number | NM_000257 |
| Gene Symbol | MYH7 |
| Gene ID (NCBI) | 4625 |
| RRID | AB_2736821 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12883 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Myosin is the major contractile protein of all eukaryotic cells, existing as a hexameric molecule composed of two myosin heavy chains (MHCs) that contain the catalytic sites for ATP hydrolysis, and four lower molecular weight myosin light chains. MYH7 encodes the slow/beta-cardiac myosin heavy chain (MHC), which is the major MHC isoform of cardiac ventricles. It is also expressed in slow, oxidative, type 1 muscle fibers of skeletal muscle. Numerous mutations of MYH7 have been linked to familial hypertrophic cardiomyopathy. This antibody can specifically recognize endogenous MYH7, not cross-reacting with other MHCs (PMID: 2523412, 14520662). Due to the production of Heavy Meromyosin (HMM) during proteolysis of myosin, a band around 150 kDa may be detected, which may correspond to the HMM part of MYH7 (PMID: 14613868).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MYH7-specific antibody 22280-1-AP | Download protocol |
| IHC protocol for MYH7-specific antibody 22280-1-AP | Download protocol |
| IP protocol for MYH7-specific antibody 22280-1-AP | Download protocol |
| WB protocol for MYH7-specific antibody 22280-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MYH7 antibody (Cat# 22280-1-AP) has accumulated 139 citations across high-impact journals including Science, Nature Communications, Circulation, Cell Reports, Developmental Cell, FASEB Journal, and Advanced Science. Research fields span cardiac hypertrophy, heart failure, cardiomyopathy, skeletal muscle fiber-type specification, sarcopenia, ferroptosis, mitochondrial dysfunction, and sarcomere biology. Applications are predominantly Western blot, with additional use in immunofluorescence and immunohistochemistry across human, mouse, rat, pig, and zebrafish models.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting intracellular cholesterol imbalance rescues sarcomere-ER contact site signaling and ER remodeling in dilated cardiomyopathy. | ||
Science HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy. | ||
Circulation Hyperglycemia-Driven Inhibition of AMP-Activated Protein Kinase α2 Induces Diabetic Cardiomyopathy by Promoting Mitochondria-Associated Endoplasmic Reticulum Membranes In Vivo. | ||
Nat Aging Multi-omic profiling of sarcopenia identifies disrupted branched-chain amino acid catabolism as a causal mechanism and therapeutic target | ||
Autophagy Cardiac fibroblast-derived CCN1 aggravates diabetic cardiomyopathy through ITGAV-ITGB1/integrin αvβ1-mediated autophagy inhibition. | ||
Nat Commun Fluorofenidone ameliorates pressure overload-induced heart failure by stabilizing SERCA2a in male mice. |
Reviews
What customers say
This product has received one review from a researcher who used it for Western Blot on hiPSC-derived cardiomyocytes at a 1:2000 dilution. The reviewer gave it a perfect 5-star rating, praising the antibody for producing strong and clear bands and stating it worked great overall.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Andrea (Verified Customer) (03-24-2025) | Strong and clear bands. Worked great!
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