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AEG-1/MTDH-Specific Polyklonaler Antikörper

AEG-1/MTDH-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13860-1-AP

Synonyme

AEG-1, MTDH, LYRIC, Astrocyte elevated gene-1 protein, AEG1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBMCF-7-Zellen, A375-Zellen, A549-Zellen, DU 145-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Mausmilzgewebe, PC-3-Zellen, RAW 264.7-Zellen
Erfolgreiche IPMCF-7-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, Maushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCMCF-7-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13860-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA AEG-1/MTDH-Specific und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AEG-1/MTDH-Specific fusion protein Ag4840
Vollständiger Name metadherin
Berechnetes Molekulargewicht 582 aa, 64 kDa
Beobachtetes Molekulargewicht 65-70 kDa
GenBank-ZugangsnummerBC045642
Gene symbol AEG1/MTDH
Gene ID (NCBI) 92140
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.

Protokolle

Produktspezifische Protokolle
WB protocol for AEG-1/MTDH-Specific antibody 13860-1-APProtokoll herunterladen
IHC protocol for AEG-1/MTDH-Specific antibody 13860-1-APProtokoll herunterladen
IF protocol for AEG-1/MTDH-Specific antibody 13860-1-APProtokoll herunterladen
IP protocol for AEG-1/MTDH-Specific antibody 13860-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIF, WB, IP, IHC

J Crohns Colitis

MTDH promotes intestinal inflammation by positively regulating TLR signaling.

Authors - Lijuan Wang
humanWB

Oncogene

MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo.

Authors - He X-X XX

Mol Ther

Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells.

Authors - Hao Teng
humanWB

Mol Ther Nucleic Acids

PMID: 28624193

Authors - Yin-Ping Fan
humanWB,IHC

Oncotarget

MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1.

Authors - Jing-Jun Yan
humanIHC

Int J Cancer

Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma.

Authors - Liu Yong Y

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

eman (Verified Customer) (11-26-2020)

this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000 and 1:2000
  • Cell Tissue Type: Hela cells
AEG-1/MTDH-Specific Antibody Western Blot,Immunoprecipitation validation (1:1000 and 1:2000 dilution) in Hela cells (Cat no:13860-1-AP)