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AEG-1/MTDH-Specific Polyklonaler Antikörper
AEG-1/MTDH-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (1)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 13860-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | MCF-7-Zellen, A375-Zellen, A549-Zellen, DU 145-Zellen, HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Mausmilzgewebe, PC-3-Zellen, RAW 264.7-Zellen |
Erfolgreiche IP | MCF-7-Zellen |
Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, Maushirngewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | MCF-7-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:200-1:800 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 12 publications below |
WB | See 41 publications below |
IHC | See 24 publications below |
IF | See 5 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
RIP | See 1 publications below |
Produktinformation
13860-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA AEG-1/MTDH-Specific und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | AEG-1/MTDH-Specific fusion protein Ag4840 |
Vollständiger Name | metadherin |
Berechnetes Molekulargewicht | 582 aa, 64 kDa |
Beobachtetes Molekulargewicht | 65-70 kDa |
GenBank-Zugangsnummer | BC045642 |
Gene symbol | AEG1/MTDH |
Gene ID (NCBI) | 92140 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MTDH, also known as AEG-1, is a single-pass transmembrane protein with its gene located at chromosome 8q22 , the abnormalities of which have been identified in several tumor types. MTDH is overexpressed in a number of malignancies, such as breast cancer, malignant glioma, and neuroblastoma , and its overexpression is associated with poor clinical outcome. MTDH plays an important role in the regulation of carcinogenesis and tumor progression. This antibody got two bands about 70 kDa and 80 kDa in western blotting, possibly due to posttranslational modification. This antibody is specific to AEG-1/MTDH.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for AEG-1/MTDH-Specific antibody 13860-1-AP | Protokoll herunterladen |
IHC protocol for AEG-1/MTDH-Specific antibody 13860-1-AP | Protokoll herunterladen |
IF protocol for AEG-1/MTDH-Specific antibody 13860-1-AP | Protokoll herunterladen |
IP protocol for AEG-1/MTDH-Specific antibody 13860-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
J Crohns Colitis MTDH promotes intestinal inflammation by positively regulating TLR signaling. | ||
Oncogene MicroRNA-375 targets AEG-1 in hepatocellular carcinoma and suppresses liver cancer cell growth in vitro and in vivo. | ||
Mol Ther Role of HCP5-miR-139-RUNX1 Feedback Loop in Regulating Malignant Behavior of Glioma Cells. | ||
Oncotarget MiR-497 suppresses angiogenesis and metastasis of hepatocellular carcinoma by inhibiting VEGFA and AEG-1. | ||
Int J Cancer Increased expression of metadherin protein predicts worse disease-free and overall survival in laryngeal squamous cell carcinoma. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH eman (Verified Customer) (11-26-2020) | this antibody works very well incubating either at room temperature or overnight2 dilutions are tested 1:1000 and 1:2000IF not tested yet
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