MCC Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MCC Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11525-1-AP

Synonyme

MCC1

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11525-1-AP

  WB result of MCC antibody (11525-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCC transfected HeLa cells.

• 

  WB analysis of fetal human brain using 11525-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11525-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11525-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11525-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11525-1-AP (MCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 11525-1-AP

  IP result of anti-MCC (IP:11525-1-AP, 3ug; Detection:11525-1-AP 1:500) with PC-3 cells lysate 5000ug.

• 

  IHC staining of human prostate cancer using 11525-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11525-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11525-1-AP (MCC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 11525-1-AP

  Immunofluorescent analysis of PC-3 cells using 11525-1-AP (MCC antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, HeLa-Zellen, humanes Hirngewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

11525-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MCC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MCC fusion protein Ag2089
• Vollständiger Name: mutated in colorectal cancers
• Berechnetes Molekulargewicht: 829 aa, 93 kDa
• Beobachtetes Molekulargewicht: 98 kDa, 113-118 kDa
• GenBank-Zugangsnummer: BC018919
• Gene symbol: MCC
• Gene ID (NCBI): 4163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,

Publikationen

Species	Application	Title
human	IF	Oncogene; 'MCC' protein interacts with E-cadherin and β-catenin strengthening cell-cell adhesion of HCT116 colon cancer cells.; Authors - F A Benthani
human	WB	Proteomics; Protein cross-talk in CD133+ colon cancer cells indicates activation of the Wnt pathway and upregulation of SRp20 that is potentially involved in tumorigenicity.; Authors - Corbo Claudia C
human,mouse	WB,IHC,IF	Front Med (Lausanne); MCC Regulator of WNT Signaling Pathway (MCC) Is a Podocyte Essential Gene.; Authors - Hui Song ; KD Validated
mouse	IHC	PLoS One.; Cancer genomics identifies regulatory gene networks associated with the transition from dysplasia to advanced lung adenocarcinomas induced by c-Raf-1.; Authors - Rohrbeck A
human	WB	bioRxiv; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan
human	WB	Nucleic Acids Res; Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes; Authors - John B Moldovan

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