MAD2L2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MAD2L2 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 12683-1-AP

Synonyme

hREV7, MAD2 like protein 2, MAD2B, MAD2L2, REV7, REV7 homolog

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Galerie der Validierungsdaten

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  WB analysis of human brain using 12683-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12683-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12683-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 12683-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12683-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12683-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12683-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 12683-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12683-1-AP (MAD2L2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12683-1-AP

  IP result of anti-MAD2L2 (IP:12683-1-AP, 3ug; Detection:12683-1-AP 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human ovary tumor using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12683-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12683-1-AP (MAD2L2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, A375-Zellen, fetale HEK-293-Zellen, HeLa-Zellen, humanes Kolongewebe, humanes Nierengewebe, K-562-Zellen, Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12683-1-AP bindet in WB, IP, IF, IHC, ELISA MAD2L2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAD2L2 fusion protein Ag3373
• Vollständiger Name: MAD2 mitotic arrest deficient-like 2 (yeast)
• Berechnetes Molekulargewicht: 211 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC015244
• Gene symbol: MAD2L2
• Gene ID (NCBI): 10459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Publikationen

Species	Application	Title
human	WB	Nat Commun; MAD2L2 dimerization and TRIP13 control shieldin activity in DNA repair.; Authors - Inge de Krijger ; KO Validated
mouse	WB	Nat Commun; SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombination.; Authors - Estelle Vincendeau
human	WB, IP	J Cell Biol; Sequestration of CDH1 by MAD2L2 prevents premature APC/C activation prior to anaphase onset.; Authors - Tamar Listovsky ; KD Validated
human	WB	Nucleic Acids Res; REV7 is essential for DNA damage tolerance via two REV3L binding sites in mammalian DNA polymerase ζ; Authors - Junya Tomida ; KD Validated
human	WB	Nucleic Acids Res; REV1 and polymerase ζ facilitate homologous recombination repair.; Authors - Sharma Shilpy S ; KD Validated
human	WB	Nucleic Acids Res; Regulation of error-prone translesion synthesis by Spartan/C1orf124.; Authors - Kim Myoung Shin MS

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