INF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

INF2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (2)

Kat-Nr. : 20466-1-AP

Synonyme

C14orf151, C14orf173, HBEBP2 binding protein C, INF2, Inverted formin 2, pp9484

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Galerie der Validierungsdaten

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  WB analysis using 20466-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20466-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20466-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20466-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 20466-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 20466-1-AP (INF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of NIH/3T3 using 20466-1-AP

  IP Result of anti-INF2 (IP:20466-1-AP, 4ug; Detection:20466-1-AP 1:500) with NIH/3T3 cells lysate 4000ug.

• 

  IHC staining of human kidney using 20466-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20466-1-AP (INF2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 20466-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20466-1-AP (INF2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 20466-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 20466-1-AP (INF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, HeLa-Zellen, K-562-Zellen, Maushirngewebe, NIH/3T3-Zellen
• Erfolgreiche IP: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 17 publications below
• CoIP: See 1 publications below

Produktinformation

20466-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA INF2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: INF2 fusion protein Ag14302
• Vollständiger Name: inverted formin, FH2 and WH2 domain containing
• Berechnetes Molekulargewicht: 1249 aa, 136 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC008756
• Gene symbol: INF2
• Gene ID (NCBI): 64423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

INF2 is a member of the formin family of actin-regulating proteins. INF2 is peripherally bound to the cytoplasmic face of the endoplasmic reticulum (ER). Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. The predicted MW of INF2 is around 136 kDa, while higher molecular mass from 140-200 kDa has been reported in literature (26383224,20493814,19366733).

Publikationen

Species	Application	Title
mouse	IF	Cell; FMN2 Makes Perinuclear Actin to Protect Nuclei during Confined Migration and Promote Metastasis.; Authors - Colleen T Skau
mouse	IF	Nat Commun; GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.; Authors - Ying Wang
human	IF	EMBO J; Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1; Authors - Santosh Phuyal
mouse	IF	Proc Natl Acad Sci U S A; Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly.; Authors - Colleen T Skau
human	WB, IF	Proc Natl Acad Sci U S A; Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim.; Authors - Xiaowei Shao ; KD Validated
zebrafish	WB,IF	Curr Biol; Calcium Wave Promotes Cell Extrusion.; Authors - Yasuto Takeuchi ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Excellent antibody. Detects a band at the correct MW.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

INF2 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:20466-1-AP)

FH

Chun (Verified Customer) (07-03-2019)

This antibody is good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293

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