IL-22RA1 Polyklonaler Antikörper

IL-22RA1 Polyklonal Antikörper für WB, IF, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA, FC (Intra)

Konjugation

Unkonjugiert

Publikationen

12

Rezensionen (2)

Kat-Nr. : 13462-1-AP

Synonyme

IL22RA1, IL22R, IL 22RA1, IL 22R alpha 1, IL 22 receptor subunit alpha 1

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Galerie der Validierungsdaten

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  WB analysis using 13462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL-22RA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13462-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13462-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13462-1-AP (IL22RA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IF Staining of human gut using 13462-1-AP

  Staining of IL22RA1 (Cat#13462-1-AP) (2ryAbisA647) on FFPE human gut. The nuclei were counter stained with DAPI (in blue). The IL22RA1 signal is localized in the epithelial compartment which is expected. (Data from Manuel Florez, Laboratory Dre. Naglaa Shoukry, CRCHUM).

• 

  FC experiment of HepG2 using 13462-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human IL-22RA1 (13462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HEK-293-Zellen, HepG2-Zellen, HT-29-Zellen, Maus-Dünndarmgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IF: human gut
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

13462-1-AP bindet in WB, IP, IF, IHC, ELISA, FC (Intra) IL-22RA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: IL-22RA1 fusion protein Ag4056
• Vollständiger Name: interleukin 22 receptor, alpha 1
• Berechnetes Molekulargewicht: 574 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63-68 kDa
• GenBank-Zugangsnummer: BC029273
• Gene symbol: IL-22RA1
• Gene ID (NCBI): 58985
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Interleukin-22 (IL-22), a member of the IL-10 family of cytokines, is important for the modulation of tissue responses during inflammation. IL-22 receptor is a heterodimer of the IL-10RB and IL-22RA1. IL-22RA1 belongs to the class II cytokine receptor family. It is expressed in a limited number of tissues including skin, colon, liver, lung, pancreas and kidney. The expression is lack in immune cells such as monocytes, T cells, and NK cells (PMID: 15308104). IL-22RA1 can also form a receptor for IL-20 and IL-24 with IL20RB (PMID: 23793061).

Publikationen

Species	Application	Title
mouse	FC	PLoS Biol; Loss of adenomatous polyposis coli function renders intestinal epithelial cells resistant to the cytokine IL-22.; Authors - Yu Chen
human	WB	Oxid Med Cell Longev; IL-22 Protects against Biliary Ischemia-Reperfusion Injury after Liver Transplantation via Activating STAT3 and Reducing Apoptosis and Oxidative Stress Levels In Vitro and In Vivo.; Authors - Yi Bai
human	WB	Clin Sci (Lond); HMGB1-induced autophagy facilitates hepatic stellate cells activation: a new pathway in liver fibrosis.; Authors - Jing Li
human	WB	Apoptosis; Elevated IL-22 in psoriasis plays an anti-apoptotic role in keratinocytes through mediating Bcl-xL/Bax.; Authors - Bo Wang
human	IHC	Biochim Biophys Acta Mol Basis Dis; IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C.; Authors - Yang Che
human	WB	Life Sci; The potential of Diosgenin in treating psoriasis: Studies from HaCaT keratinocytes and imiquimod-induced murine model.; Authors - Shidi Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Iram (Verified Customer) (06-13-2022)

Very good antibody for my western blotting

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: colon cancer cells

FH

Susmita (Verified Customer) (03-11-2022)

Excellent antibody

• Applications: Western Blot
• Primary Antibody Dilution: WB: 1: 2000
• Cell Tissue Type: PC cell line

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