ST2 Monoklonaler Antikörper

ST2 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

CloneNo.

7A2A7

Kat-Nr. : 60112-1-Ig

Synonyme

DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1

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Galerie der Validierungsdaten

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  WB analysis using 60112-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60112-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60112-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 60112-1-Ig

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 60112-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60112-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 60112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 60112-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60112-1-Ig (ST2 antibody at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HEK-293 using 60112-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20ug ST2 antibody (60112-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, fetales humanes Hirngewebe, Hausschwein-Hirngewebe, Maushirngewebe, PC-12-Zellen, Ratten-Cerebellum-Gewebe, Rattenhirngewebe, ROS1728-Zellen, SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Produktinformation

60112-1-Ig bindet in WB, IHC, IF, FC, ELISA ST2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ST2 fusion protein Ag2555
• Vollständiger Name: interleukin 1 receptor-like 1
• Berechnetes Molekulargewicht: 63 kDa, 37 kDa, 30 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC030975
• Gene symbol: IL1RL1
• Gene ID (NCBI): 9173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Publikationen

Species	Application	Title
mouse	IF	Immunity; Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis.; Authors - Zuojia Chen
human	WB,IHC	Sci Rep; Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression.; Authors - Elena Vianello
human	IF	Sci Rep; Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model.; Authors - Shuhei Kobayashi
mouse	IF	Cancer Biomark; Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model; Authors - Shan Wang
human	IF, WB	Inflamm Res; Molecular hydrogen alleviates asthma through inhibiting IL-33/ILC2 axis.; Authors - Jingxi Zhang
pig	IHC	Am J Physiol Renal Physiol; INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS.; Authors - Alejandro R Chade

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