ICAM-1 Polyklonaler Antikörper

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• KD/KO Validated

ICAM-1 Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15364-1-AP

Synonyme

BB2, CD54, ICAM 1, ICAM1, P3.58

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Galerie der Validierungsdaten

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  WB analysis of Raji using 15364-1-AP

  WB result of ICAM1 antibody (15364-1-AP, 1:20,000) with si-Control and si-ICAM1 transfected Raji cells. .

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  WB analysis of human heart using 15364-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15364-1-AP (ICAM-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15364-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15364-1-AP (ICAM-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 15364-1-AP

  IP Result of anti-ICAM-1 (IP:15364-1-AP, 4ug; Detection:15364-1-AP 1:500) with Raji cells lysate 1520ug.

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  IHC staining of human colon cancer using 15364-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15364-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 15364-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15364-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15364-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 15364-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15364-1-AP (ICAM-1 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of Raji using 15364-1-AP

  1X10^6 Raji cells were stained with 0.2ug ICAM-1 antibody (15364-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, Raji-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15364-1-AP bindet in WB, IP, IHC, IF, FC, ELISA ICAM-1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ICAM-1 fusion protein Ag8309
• Vollständiger Name: intercellular adhesion molecule 1
• Berechnetes Molekulargewicht: 90 kDa
• Beobachtetes Molekulargewicht: 85-95 kDa
• GenBank-Zugangsnummer: BC015969
• Gene symbol: ICAM1
• Gene ID (NCBI): 3383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes¹. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-1². In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions^{3, 4}. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material⁵.

1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003). 

Publikationen

Species	Application	Title
human	IF	Acta Pharm Sin B; Pulmonary endothelium-targeted nanoassembly of indomethacin and superoxide dismutase relieves lung inflammation; Authors - Yi Yang
human	WB	Curr Pharm Biotechnol; Naringin inhibits TNF-α induced oxidative stress and inflammatory response in HUVECs via Nox4/NF-κ B and PI3K/Akt pathways.; Authors - Wenshuang Li
human	IHC	J Mol Histol; Prognostic prediction and diagnostic role of intercellular adhesion molecule-1 (ICAM1) expression in clear cell renal cell carcinoma.; Authors - Xuebing Shi

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