- Featured Product
- KD/KO Validated
IBA1 Polyklonaler Antikörper
IBA1 Polyklonal Antikörper für IHC, IF-P, FC (Intra), ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (3)
Anwendung
IHC, IF-P, FC (Intra), ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 10904-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche IP | THP-1-Zellen |
| Erfolgreiche Detektion in IHC | Rattenhirngewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Tonsillitisgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF-P | Maushirngewebe, Rattenhirngewebe |
| Erfolgreiche Detektion in FC (Intra) | THP-1-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunhistochemie (IHC) | IHC : 1:400-1:1600 |
| Immunfluoreszenz (IF)-P | IF-P : 1:50-1:500 |
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 5 publications below |
| IHC | See 94 publications below |
| IF | See 207 publications below |
Produktinformation
10904-1-AP bindet in IHC, IF-P, FC (Intra), ELISA IBA1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Maus, Ratte, Zebrafisch |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | IBA1 fusion protein Ag1363 |
| Vollständiger Name | allograft inflammatory factor 1 |
| Berechnetes Molekulargewicht | 17 kDa |
| GenBank-Zugangsnummer | BC009474 |
| Gene symbol | IBA1 |
| Gene ID (NCBI) | 199 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
What is the molecular weight of IBA1?
The molecular weight of IBA1 is 16.7 kD.
What is IBA1?
Ionized calcium-binding adaptor molecule 1 (IBA1), also known as Allograft inflammatory factor-1 (AIF-1), is an inflammation-responsive scaffold protein expressed and secreted from macrophages. Microglia response factor (MRF-1) and daintain are also similar, and likely identical, proteins (PMIDs: 29749461, 9630473, 23792284).
What is the function of IBA1?
IBA1 is necessary for macrophage survival, and it is also a key molecule in proinflammatory activity (PMID: 29749461).
Where is IBA1 localized?
IBA1 is a cytoplasmic protein, often expressed in immunocytes, macrophages, and microglia. It can be used as a marker for normal (not 'dark') microglia in brain tissue, as IBA1 is expressed by all microglial cell subpopulations (PMIDs: 29749461, 11943136, 26847266, 9630473).
Is IBA1 upregulated in active immunophages?
Yes; its expression is associated with inflammatory activity (PMID: 29749461).
How do IBA1-positive microglia differ from microglia that express less or no IBA1?
'Dark' microglia is a recently described phenotype associated with Alzheimer's disease pathology and chronic stress; these dark microglia express decreased IBA1 (often punctiform), and show distinct ultrastructural differences from 'normal' microglia as well as condensed and electron-dense cytoplasm and nucleoplasm. Normal microglia generally display strong, diffuse expression of IBA1. IBA1-positive microglial processes in normal conditions also have a strong tendency to exclusively contact synaptic elements, such as axon terminals, dendritic spines, and synaptic clefts (PMIDs: 29992181, 26847266).
How is IBA1 related to Alzheimer's disease and pain?
Exacerbated immunoactivity of IBA1, particularly in proximity to amyloid plaques, is prominently featured in AD pathology. IBA1, along with other microglial markers, are also associated with pain, and robust microglial reactions often follow spinal cord injury (PMIDs: 29992181, 23792284).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| IHC protocol for IBA1 antibody 10904-1-AP | Protokoll herunterladen |
| IF protocol for IBA1 antibody 10904-1-AP | Protokoll herunterladen |
| IP protocol for IBA1 antibody 10904-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Int J Surg Bioinformatics analysis identifies CSF1R as an essential gene mediating Neuropathic pain - Experimental research. | ||
Brain MMP13 inhibition rescues cognitive decline in Alzheimer transgenic mice via BACE1 regulation. | ||
Brain Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2. | ||
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (04-05-2024) | Iba1 (in green) marks clearly my microglia in human brain FFPE cortex.
 |
FH Alexandru (Verified Customer) (11-13-2023) | Very pleased with the antibody! I had a claer signal in all my stains.
|
FH NX (Verified Customer) (02-27-2023) | A clean antibody to detect endogenous Iba1 from mouse brain lysate.
|
