GPRIN1 Polyklonaler Antikörper

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• KD/KO Validated

GPRIN1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13771-1-AP

Synonyme

GPRIN1, GRIN1, KIAA1893

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13771-1-AP (GPRIN1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13771-1-AP (GPRIN1 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13771-1-AP

  IP result of anti-GPRIN1 (IP:13771-1-AP, 3ug; Detection:13771-1-AP 1:500) with mouse brain tissue lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

13771-1-AP bindet in WB, IP, IF, IHC, ELISA GPRIN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GPRIN1 fusion protein Ag4754
• Vollständiger Name: G protein regulated inducer of neurite outgrowth 1
• Berechnetes Molekulargewicht: 1008 aa, 102 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC052950
• Gene symbol: GPRIN1
• Gene ID (NCBI): 114787
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPRIN1 (G protein-regulated inducer of neurite outgrowth 1), also named GRIN1, may be involved in neurite outgrowth. Western blotting analysis has confirmed its expression in mouse neuroblastoma and rat pheochromocytoma cells. And both GPRIN1 and GNAO1 are membrane-bound proteins that are enriched in the growth cones of neurites (PMID:10480904). It has 2 isoforms produced by alternative splicing. This antibody detects GRIN1 at bands around 110 kDa.

Publikationen

Species	Application	Title
mouse	WB,IHC,IF	Sci Signal; Dynamic interactions of the 5-HT6 receptor with protein partners control dendritic tree morphogenesis.; Authors - Camille N Pujol ; KD Validated
mouse	WB	J Biol Chem; DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory.; Authors - Li Yi Y
human	WB	Exp Ther Med; Chronic ethanol exposure induces SK-N-SH cell apoptosis by increasing N-methyl-D-aspartic acid receptor expression and intracellular calcium.; Authors - Hongbo Wang

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