GNAO1 Polyclonal antibody

GNAO1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 12635-1-AP

Synonyms

G ALPHA o, GNAO, GNAO1

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Validation Data Gallery

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  WB analysis using 12635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12635-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 12635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12635-1-AP (GNAO1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12635-1-AP

  IP result of anti-GNAO1 (IP:12635-1-AP, 3ug; Detection:12635-1-AP 1:600) with mouse brain tissue lysate 3600ug.

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  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12635-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12635-1-AP (GNAO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, Y79 cells, human liver tissue, mouse testis tissue, rat testis tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

12635-1-AP targets GNAO1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GNAO1 fusion protein Ag3330
• Full Name: guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O
• Calculated Molecular Weight: 302 aa, 35 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC030027
• Gene Symbol: GNAO1
• Gene ID (NCBI): 2775
• RRID: AB_2111635
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GNAO1 gene encodes the α subunit of heterotrimeric guanine nucleotide-binding protein (Gi protein), a membrane protein widely expressed in the central nervous system involved in signal transduction (PMID: 30642806). GNAO1 belongs to the G-alpha family and G(i/o/t/z) subfamily. GNAO1 is highly expressed in brain and modulate neuronal excitability (PMID: 30838255). GNAO1 has 2 isoforms with the molecular mass of 40 kDa.

Publications

Species	Application	Title
human	WB	Blood; Identification of functional cooperative mutations of GNAO1 in human acute lymphoblastic leukemia.; Authors - Lili Song
mouse	IF	Elife; Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis; Authors - Amandine Jarysta
human	WB	Neurochem Res; Efficacy of Hybrid Tetrahydrobenzo[d]thiazole Based Aryl Piperazines D-264 and D-301 at D₂ and D₃ Receptors.; Authors - Juan Zhen
human	WB	Gene; DNMT1 mediated promoter methylation of GNAO1 in hepatoma carcinoma cells.; Authors - Diannan Xu
human	IHC	Int J Mol Med; Overexpression of GNAO1 correlates with poor prognosis in patients with gastric cancer and plays a role in gastric cancer cell proliferation and apoptosis.; Authors - Zhen Liu
human	IHC	Biosci Rep; The down-regulation of GNAO1 and its promoting role in hepatocellular carcinoma.; Authors - Pei Xiaoyu X