GJB3 Polyklonaler Antikörper

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• KD/KO Validated

GJB3 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 12880-1-AP

Synonyme

EKV, DFNA2B, DFNA2, CX31, Connexin-31

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12880-1-AP

  WB result of GJB3 antibody (12880-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GJB3 transfected HeLa cells.

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  WB analysis using 12880-1-AP

  mouse stomach tissue was subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 12880-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12880-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12880-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12880-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12880-1-AP (GJB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12880-1-AP

  IP result of anti-GJB3 (IP:12880-1-AP, 4ug; Detection:12880-1-AP 1:500) with HeLa cells lysate 2600 ug.

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  IHC staining of human cervix using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse placenta using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12880-1-AP (GJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse placenta using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12880-1-AP (GJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin cancer using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12880-1-AP (GJB3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 12880-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12880-1-AP (GJB3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of human placenta using 12880-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using GJB3 antibody (12880-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Magengewebe, HeLa-Zellen, Maus-Eierstockgewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Plazenta-Gewebe, humanes Hautkrebsgewebe, humanes Ovarialkarzinomgewebe, humanes Zervixgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Produktinformation

12880-1-AP bindet in WB, IHC, IF-P, IP, ELISA GJB3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GJB3 fusion protein Ag3839
• Vollständiger Name: gap junction protein, beta 3, 31kDa
• Berechnetes Molekulargewicht: 270 aa, 31 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC012918
• Gene symbol: GJB3
• Gene ID (NCBI): 2707
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GJB3, also known as Connexin-31 (Cx31), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Mutations in the gene of GJB3 have been described in patients with dominant and recessive hearing impairment and in patients with erythrokeratodermia variabilis (EKV).

Publikationen

Species	Application	Title
human	WB	Cell Rep; Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss.; Authors - Makoto Hosoya
mouse	IHC	Stem Cells; FBXL12-Mediated Degradation of ALDH3 Is Essential for Trophoblast Differentiation during Placental Development.; Authors - Masaaki Nishiyama
mouse	IF	J Neurosci; Glial-Specific Deletion of Med12 Results in Rapid Hearing Loss via Degradation of the Stria Vascularis.; Authors - Teng-Wei Huang
marmoset	IF	Sci Rep; Distinct Expression Patterns Of Causative Genes Responsible For Hereditary Progressive Hearing Loss In Non-Human Primate Cochlea.; Authors - Makoto Hosoya
mouse	IF	Placenta; In vitro fertilization causes excessive glycogen accumulation in mouse placenta.; Authors - Jie Dong
mouse	IHC	J Pain Res; PMID: 29692624; Authors - Brice Bellessort

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