Connexin-32 Polyclonal antibody

Connexin-32 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 10450-1-AP

Synonyms

GJB1, Gap junction beta-1 protein, Gap junction beta 1 protein, CX32, CMTX1

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Validation Data Gallery

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  WB analysis of mouse brain using 10450-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 10450-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 10450-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10450-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10450-1-AP (GJB1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10450-1-AP

  IP result of anti-Connexin-32 (IP:10450-1-AP, 4ug; Detection:10450-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10450-1-AP (Connexin-32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10450-1-AP (Connexin-32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (GJB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10450-1-AP (GJB1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A375 cells, human liver tissue, mouse skin tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

10450-1-AP targets Connexin-32 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Connexin-32 fusion protein Ag0659
• Full Name: gap junction protein, beta 1, 32kDa
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC002805
• Gene Symbol: Connexin-32
• Gene ID (NCBI): 2705
• RRID: AB_2110629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Connexin-32 (Cx32), also known as Gap junction beta 1 protein (GJB1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. Connexin-32 is the major gap junction protein of liver, and the expression is also found in the brain and other organs. It is involved in tumorigenesis and liver regeneration. Connexin-32 plays an important role in peripheral nerve. Defects in Connexin-32 are the cause of Charcot-Marie-Tooth disease X-linked type 1 (CMTX1), an inherited peripheral neuropathy. Cx32 protein can be detected as a 27-32 kDa monomer and a 48-54 kDa dimer (PMID: 8266101; 8613761; 8608591; 19265674; 17972320; 19845480)

Publications

Species	Application	Title
human	WB	Chemosphere; Organophosphate flame retardant TDCPP: A risk factor for renal cancer?; Authors - Xuan Zhou
human	WB	World J Gastroenterol; PBX1 attributes as a determinant of connexin 32 downregulation in Helicobacter pylori-related gastric carcinogenesis.; Authors - Xiao-Ming Liu
mouse	WB	Brain Res Bull; Transient receptor potential vanilloid 4 is involved in the upregulation of connexin expression following pilocarpine-induced status epilepticus in mice.; Authors - Chen Men
human	IHC	J Biomed Biotechnol; Ischemia alters the expression of connexins in the aged human brain.; Authors - Nakase Taizen T
human	IHC, IF	Histopathology; Down-regulation of connexin43 and connexin32 in keratocystic odontogenic tumours: potential association with clinical features.; Authors - Wen-Qun Zhong
human	WB	Oncol Lett; Effects of Helicobacter pylori on the expression levels of GATA-3 and connexin 32 and the GJIC function in gastric epithelial cells and their association by promoter analysis.; Authors - Lihua Huang

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