Connexin-43 Polyklonaler Antikörper

Connexin-43 Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Kat-Nr. : 15386-1-AP

Synonyme

Connexin-43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 15386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15386-1-AP (GJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15386-1-AP

  IP Result of anti-Connexin-43 (IP:15386-1-AP, 4ug; Detection:15386-1-AP 1:500) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15386-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 15386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 15386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 15386-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug Connexin-43 antibody (15386-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 17 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15386-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, ELISA Connexin-43 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Connexin-43 fusion protein Ag6698
• Vollständiger Name: gap junction protein, alpha 1, 43kDa
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-47 kDa
• GenBank-Zugangsnummer: BC026329
• Gene symbol: GJA1
• Gene ID (NCBI): 2697
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Publikationen

Species	Application	Title
human	IF	Nat Commun; Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.; Authors - Yin Wang
mouse	IF	Thromb Res; Selective increase of cardiomyocyte derived extracellular vesicles after experimental myocardial infarction and functional effects on the endothelium.; Authors - Jose A Rodriguez
mouse	WB	Hypertension; Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.; Authors - Jing Li
	IF	Ecotoxicol Environ Saf; Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage.; Authors - E O Adegoke
human	WB	Environ Toxicol Pharmacol; Microcystin-leucine arginine (MC-LR) induced inflammatory response in bovine sertoli cell via TLR4/NF-kB signaling pathway.; Authors - E O Adegoke
human	IF	Free Radic Biol Med; Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells.; Authors - Justyna Mikuła-Pietrasik

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