Galectin-1 Polyklonaler Antikörper

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• KD/KO Validated

Galectin-1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 11858-1-AP

Synonyme

GAL1, Gal 1, Beta-galactoside-binding lectin L-14-I, 14 kDa lectin, 14 kDa laminin-binding protein

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 11858-1-AP

  WB result of Galectin-1 antibody (11858-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin-1 transfected HepG2 cells.

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  WB analysis using 11858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11858-1-AP (Galectin-1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11858-1-AP (Galectin-1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11858-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11858-1-AP (Galectin-1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 11858-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Galectin-1 antibody (11858-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HCT 116-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:200-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

11858-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Galectin-1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-1 fusion protein Ag2432
• Vollständiger Name: lectin, galactoside-binding, soluble, 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC020675
• Gene symbol: Galectin-1
• Gene ID (NCBI): 3956
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-1 contains one carbohydrate recognition domain (CRD) and occurs as a monomer as well as a non-covalent homodimer (PMID: 16840800). It is differentially expressed by various normal and pathological tissues. Galectin-1 is a multifunctional protein that is involved in cell adhesion, migration, proliferation, apoptosis, inflammation, tumour transformation and growth (PMID:15785741).

Publikationen

Species	Application	Title
human	WB	Cell Rep; Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.; Authors - Krishna S Tummala
human	WB	Mol Ther Nucleic Acids; Circular RNA RBPMS inhibits bladder cancer progression via miR-330-3p/RAI2 regulation.; Authors - Chen Yang
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
mouse	IHC	Biomed Pharmacother; Mesenchymal stem cells target microglia via galectin-1 production to rescue aged mice from olfactory dysfunction.; Authors - Yoojin Seo
human	IHC	Int J Oncol; Galectin‑1 binds GRP78 to promote the proliferation and metastasis of gastric cancer; Authors - Qi Zhang
mouse	IHC	Front Med (Lausanne); An Integrated Proteomics and Metabolomics Strategy for the Mechanism of Calcium Oxalate Crystal-Induced Kidney Injury.; Authors - Songyan Gao

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