FRS2 Polyklonaler Antikörper

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• KD/KO Validated

FRS2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 11503-1-AP

Synonyme

SNT 1, SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT

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Galerie der Validierungsdaten

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  WB analysis using 11503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11503-1-AP

  WB result of FRS2 antibody (11503-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FRS2 transfected SH-SY5Y cells.

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  WB analysis of human brain using 11503-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11503-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11503-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11503-1-AP (FRS2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 11503-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11503-1-AP (FRS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, NIH/3T3-Zellen, PC-3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below

Produktinformation

11503-1-AP bindet in WB, IHC, IF/ICC, ELISA FRS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FRS2 fusion protein Ag2052
• Vollständiger Name: fibroblast growth factor receptor substrate 2
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC021562
• Gene symbol: FRS2
• Gene ID (NCBI): 10818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

Publikationen

Species	Application	Title
	WB	Cancer Res; Transcriptomics and transposon mutagenesis identify multiple mechanisms of resistance to the FGFR inhibitor AZD4547.; Authors - Sjors M Kas
human	IHC	Cancer Res; Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma.; Authors - Zhang Keqiang K
mouse	WB	J Med Chem; Design, Synthesis, and Biological Evaluation of Aminoindazole Derivatives as Highly Selective Covalent Inhibitors of Wild-Type and Gatekeeper Mutant FGFR4.; Authors - Min Shao
mouse	WB	Cells; FGF9, a Potent Mitogen, Is a New Ligand for Integrin αvβ3, and the FGF9 Mutant Defective in Integrin Binding Acts as an Antagonist; Authors - Chih-Chieh Chang
human	WB	Breast Cancer Res; FGFR1 amplification or overexpression and hormonal resistance in luminal breast cancer: rationale for a triple blockade of ER, CDK4/6, and FGFR1.; Authors - Silvana Mouron
human	WB	Genes Chromosomes Cancer; High-resolution genomic mapping reveals consistent amplification of the fibroblast growth factor receptor substrate 2 gene in well-differentiated and dedifferentiated liposarcoma.; Authors - Wang Xiaoke X

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