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Fibromodulin Polyklonaler Antikörper

Fibromodulin Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13281-1-AP

Synonyme

FMOD, SLRR2E, KSPG fibromodulin, Keratan sulfate proteoglycan fibromodulin, FM

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human liver tissue using Fibromodulin Polyclonal antibody (13281-1-AP)

    WB analysis of human liver using 13281-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13281-1-AP (Fibromodulin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Fibromodulin Polyclonal antibody (13281-1-AP)

    IF Staining of HeLa using 13281-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13281-1-AP (Fibromodulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumanes Lebergewebe
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below

Produktinformation

13281-1-AP bindet in WB, IF/ICC, ELISA Fibromodulin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Fibromodulin fusion protein Ag4030
Vollständiger Name fibromodulin
Berechnetes Molekulargewicht 376 aa, 43 kDa
Beobachtetes Molekulargewicht 43 kDa
GenBank-ZugangsnummerBC035281
Gene symbol Fibromodulin
Gene ID (NCBI) 2331
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).

Protokolle

Produktspezifische Protokolle
WB protocol for Fibromodulin antibody 13281-1-APProtokoll herunterladen
IF protocol for Fibromodulin antibody 13281-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

iScience

Fibromodulin overexpression drives oral squamous cell carcinoma via activating downstream EGFR signaling

Authors - Lingyun Xia
View Article