FGFR4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FGFR4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 11098-1-AP

Synonyme

TKF, JTK2, FGFR-4, FGFR 4, EC:2.7.10.1

---

Galerie der Validierungsdaten

• 

  WB analysis of HepG2 using 11098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11098-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11098-1-AP (FGFR4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11098-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11098-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11098-1-AP (FGFR4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11098-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 11098-1-AP (FGFR4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Produktinformation

11098-1-AP bindet in WB, IHC, IF/ICC, ELISA FGFR4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FGFR4 fusion protein Ag1573
• Vollständiger Name: fibroblast growth factor receptor 4
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC011847
• Gene symbol: FGFR4
• Gene ID (NCBI): 2264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fibroblast growth factor receptor 4 (FGFR4) is a member of a highly conserved tyrosine kinase family, along with FGFR1-3. This family consists of an intracellular tyrosine kinase domain, a single transmembrane domain, and extracellular ligand binding domains (PMID:32492514). FGFR4 is the predominant FGFR isoform present in human hepatocytes. FGFR4 has been proposed to play a role in the observed induction of hepatocyte proliferation and carcinogenesis by FGF19; however, contradicting evidence proposing a protective role for FGFR4 in suppressing hepatoma progression has also been proposed(PMID:20018895). While the role of FGFR4 in cancer remains to be fully elucidated, several findings suggest that this receptor may be an important player in Hepatocellular carcinoma (HCC) development and/or progression(PMID:10336501).

Publikationen

Species	Application	Title
human,mouse	WB,IHC	J Med Chem; FGF19-Based Mini Probe Targeting FGFR4 for Diagnosis and Surgical Navigation of Hepatocellular Carcinoma; Authors - Jiaxian Cai ; KD Validated
mouse	WB	Biochem Pharmacol; Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity.; Authors - Yanan Chen
mouse	WB	Biomed Pharmacother; Bupleuri radix extract ameliorates impaired lipid metabolism in high-fat diet-induced obese mice via gut microbia-mediated regulation of FGF21 signaling pathway.; Authors - Liucen Wu
human		J Cell Biochem; Glycosylation of FGFR4 in cholangiocarcinoma regulates receptor processing and cancer signaling.; Authors - Andrew J Phillips
human	IHC, WB	Int J Oncol; Role of fibroblast growth factor 4 in the growth and metastasis of colorectal cancer.; Authors - Yanwei Ye
human	IHC	Cancer Res Treat; FGFR4 Gly388Arg Polymorphism Affects the Progression of Gastric Cancer by Activating STAT3 Pathway to Induce Epithelial to Mesenchymal Transition.; Authors - Yanwei Ye

Verwandte Produkte

FGFR1 Monoclonal antibody

60325-1-Ig

FGFR2 Polyclonal antibody

13042-1-AP

FRS2 Polyclonal antibody

11503-1-AP

NUDT6 Polyclonal antibody

11181-1-AP