FRS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FRS2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 11503-1-AP

Synonyms

SNT 1, SNT, FRS2alpha, FRS2A, FGFR-signaling adaptor SNT

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Validation Data Gallery

• 

  WB analysis using 11503-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 11503-1-AP

  WB result of FRS2 antibody (11503-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FRS2 transfected SH-SY5Y cells.

• 

  WB analysis of NIH/3T3 using 11503-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11503-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11503-1-AP (FRS2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11503-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11503-1-AP (FRS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 11503-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11503-1-AP (FRS2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells, human brain tissue, NIH/3T3 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below

Product Information

11503-1-AP targets FRS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FRS2 fusion protein Ag2052
• Full Name: fibroblast growth factor receptor substrate 2
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC021562
• Gene Symbol: FRS2
• Gene ID (NCBI): 10818
• RRID: AB_2262942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor (FGF) receptor substrate 2 (FRS2) has an alternative name as SNT-1, it is an adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. FGF receptor substrates (FRS2 and FRS3) are key adaptor proteins that mediate FGF-FGFR signalling in benign as well as malignant tissue. FRS2 is a 508 amino-acid protein,which is phosphorylated on tyrosine residues. The molecular weight of non-phosphorylated FRS2 is 57-68 kDa, but phosphorylated FRS2 is 80-90 kDa. Phosphorylation of FRS2 is associated with activation of a number of MAP kinases. Allele-specific regulation of FGFR2 mRNA expression with a mildly increased breast cancer risk has been reported.

Publications

Species	Application	Title
	WB	Cancer Res; Transcriptomics and transposon mutagenesis identify multiple mechanisms of resistance to the FGFR inhibitor AZD4547.; Authors - Sjors M Kas
human	IHC	Cancer Res; Amplification of FRS2 and activation of FGFR/FRS2 signaling pathway in high-grade liposarcoma.; Authors - Zhang Keqiang K
mouse	WB	J Med Chem; Design, Synthesis, and Biological Evaluation of Aminoindazole Derivatives as Highly Selective Covalent Inhibitors of Wild-Type and Gatekeeper Mutant FGFR4.; Authors - Min Shao
mouse	WB	Cells; FGF9, a Potent Mitogen, Is a New Ligand for Integrin αvβ3, and the FGF9 Mutant Defective in Integrin Binding Acts as an Antagonist; Authors - Chih-Chieh Chang
human	WB	Breast Cancer Res; FGFR1 amplification or overexpression and hormonal resistance in luminal breast cancer: rationale for a triple blockade of ER, CDK4/6, and FGFR1.; Authors - Silvana Mouron
human	WB	Genes Chromosomes Cancer; High-resolution genomic mapping reveals consistent amplification of the fibroblast growth factor receptor substrate 2 gene in well-differentiated and dedifferentiated liposarcoma.; Authors - Wang Xiaoke X

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