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  • KD/KO Validated

ESRRB Polyklonaler Antikörper

ESRRB Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22644-1-AP

Synonyme

DFNB35, ERR2, ERRb, ERRbeta, ERRbeta 2, ESRL2, ESRRB, ESRRB protein, estrogen related receptor beta, NR3B2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBPC-13-Zellen, A549-Zellen
Erfolgreiche IPA549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22644-1-AP bindet in WB, IP, IHC, IF, ELISA ESRRB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen ESRRB fusion protein Ag18523
Vollständiger Name estrogen-related receptor beta
Berechnetes Molekulargewicht 508 aa, 56 kDa
Beobachtetes Molekulargewicht 48-56 kDa
GenBank-ZugangsnummerBC131517
Gene symbol ESRRB
Gene ID (NCBI) 2103
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.

Protokolle

Produktspezifische Protokolle
WB protocol for ESRRB antibody 22644-1-APProtokoll herunterladen
IP protocol for ESRRB antibody 22644-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
mouseIF

Nucleic Acids Res

The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity.

Authors - Stephanie Chrysanthou
humanWB,IF

Genes Dev

Role of the small subunit processome in the maintenance of pluripotent stem cells.

Authors - Kwon Tae You
mouseWB,IF

Front Cell Dev Biol

ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.

Authors - Shuai Yu
  • KD Validated
mouseWB

ACS Nano

Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation.

Authors - Jie Zhang
humanWB

Nat Commun

Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.

Authors - J Mathieu
  • KO Validated