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ESRRB Polyklonaler Antikörper
ESRRB Polyklonal Antikörper für IP, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 22644-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | PC-13-Zellen, A549-Zellen |
Erfolgreiche IP | A549-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 2 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
Produktinformation
22644-1-AP bindet in WB, IP, IHC, IF, ELISA ESRRB und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | ESRRB fusion protein Ag18523 |
Vollständiger Name | estrogen-related receptor beta |
Berechnetes Molekulargewicht | 508 aa, 56 kDa |
Beobachtetes Molekulargewicht | 48-56 kDa |
GenBank-Zugangsnummer | BC131517 |
Gene symbol | ESRRB |
Gene ID (NCBI) | 2103 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Estrogen related receptor beta (ESRRB), a NR3 Steroid Receptor, has been shown to affect early placental development. Also it can act as a repressor of transcriptional activity mediated by the glucocorticoid receptor (GR). It binds as a monomer to the extended half-site TNAAGTGCA and as a homodimer to the estrogen response element (ERE), palindromic thyroid hormone response element (TRE(pal)), and SF-1 response element, but not to the glucocorticoid response element (GRE). Estrogen related receptor beta mutations lead to abnormal development of the chorion and defective diploid trophoblast proliferation, and are lethal during midgestation. Expression has been documented in mice in the extra embryonic ectoderm that gives rise to the chorion. ESTs have been isolated from normal human eye, lung, and testis libraries. The ligand for the receptor is PPARgamma coactivator 1 (PGC-1) beta. ESRRB exists various isoforms and molecular weight of each isoform is 48 kDa,55 kDa and 56 kDa.
Protokolle
Produktspezifische Protokolle | |
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WB protocol for ESRRB antibody 22644-1-AP | Protokoll herunterladen |
IP protocol for ESRRB antibody 22644-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Nucleic Acids Res The DNA dioxygenase Tet1 regulates H3K27 modification and embryonic stem cell biology independent of its catalytic activity. | ||
Genes Dev Role of the small subunit processome in the maintenance of pluripotent stem cells. | ||
Front Cell Dev Biol ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.
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ACS Nano Silver Nanoparticles Compromise Female Embryonic Stem Cell Differentiation through Disturbing X Chromosome Inactivation. | ||
Nat Commun Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.
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