ESRRG Polyclonal antibody

• Featured Product
• KD/KO Validated

ESRRG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14017-1-AP

Synonyms

ERR gamma 2, ERR3, ERRG2, ERRgamma, ESRRG, estrogen related receptor gamma, KIAA0832, NR3B3

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Validation Data Gallery

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  WB analysis of human spleen using 14017-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 14017-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14017-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14017-1-AP (ESRRG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human spleen tissue, human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14017-1-AP targets ESRRG in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESRRG fusion protein Ag5127
• Full Name: estrogen-related receptor gamma
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC064700
• Gene Symbol: ESRRG
• Gene ID (NCBI): 2104
• RRID: AB_2100272
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen-related receptor gamma (ERR-gamma), also known as NR3B3 (nuclear receptor subfamily 3, group B, member 3), is a member of nuclear hormone receptor family of steroid hormone receptors. As an orphan nuclear receptor, it binds specifically to an estrogen response element and activates reporter genes controlled by estrogen response elements. It behaves as a constitutive activator of transcription. 4-hydroxytamoxifen and diethylstilbestrol may act as inverse agonists and deactivate ESRRG. It also seems to be the target of bisphenol A. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human ESRRG.

Publications

Species	Application	Title
human	IHC,IF	Acta Neuropathol Commun; Nucleolar stress in C9orf72 and sporadic ALS spinal motor neurons precedes TDP-43 mislocalization.; Authors - Olubankole Aladesuyi Arogundade
human	WB	J Transl Med; ESRRG-PKM2 axis reprograms metabolism to suppress esophageal squamous carcinoma progression and enhance anti-PD-1 therapy efficacy; Authors - Tianxiao Wang
human	WB	Mol Cancer Res; Integrative Epigenetic and Gene Expression Analysis of Renal Tumor Progression to Metastasis.; Authors - Hye-Young Nam
mouse	WB	Int J Biochem Cell Biol; Estrogen-related receptor (ERR) γ protects against puromycin aminonucleoside-induced podocyte apoptosis by targeting PI3K/Akt signaling.; Authors - Wei Gong ; KD Validated