HER2/ErbB2 Polyklonaler Antikörper
HER2/ErbB2 Polyklonal Antikörper für IF, IHC, WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, IHC, IF,ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 51105-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, MCF-7-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe, Breast Cancer slides Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunhistochemie (IHC) | IHC : 1:20-1:200 |
| Immunfluoreszenz (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
51105-1-AP bindet in WB, IHC, IF,ELISA HER2/ErbB2 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| Berechnetes Molekulargewicht | 185 kDa |
| Beobachtetes Molekulargewicht | 185 kDa |
| GenBank-Zugangsnummer | BC167147 |
| Gene symbol | ERBB2 |
| Gene ID (NCBI) | 2064 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20℃ lagern. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for HER2/ErbB2 antibody 51105-1-AP | Protokoll herunterladen |
| IHC protocol for HER2/ErbB2 antibody 51105-1-AP | Protokoll herunterladen |
| IF protocol for HER2/ErbB2 antibody 51105-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Wei (Verified Customer) (11-26-2018) | We found two bands between 250 and 150kDa and they are very close to each other. The lower band is the right band but the topper band is too dominant in different breast cancer cells we detected.
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