ERBB4 Polyclonal antibody

ERBB4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 19943-1-AP

Synonyms

p180erbB4, HER4, ERBB4 intracellular domain, EC:2.7.10.1, E4ICD

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Validation Data Gallery

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  WB analysis of mouse brain using 19943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19943-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19943-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 19943-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 19943-1-AP (ERBB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse lung using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 19943-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19943-1-AP (ERBB4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse heart using 19943-1-AP

  Frozen tissue section of embryonic mouse heart (E12.5) was stained for cardiac troponin-T/cTnT (green) and ErbB4 (red, Cat. No 19943-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). cTnT stains cardiomyocytes and was visualized with an Alexa Fluor 488 secondary antibody. ErbB4 is a key member of ErbB signaling and involved in development and was visualized with an Alexa Fluor 555 secondary antibody. The Image and figure legends are intellectual property of @LAF_in_the_LAB.

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells, rat brain tissue
• Positive IHC detected in: mouse lung tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

19943-1-AP targets ERBB4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
• Calculated Molecular Weight: 147 kDa
• Observed Molecular Weight: 180 kDa, 147 kDa
• GenBank Accession Number: NM_001042599
• Gene Symbol: ERBB4
• Gene ID (NCBI): 2066
• RRID: AB_10646486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERBB4, also named p180erbB4 and HER4, belongs to the protein kinase superfamily, Tyr protein kinase family, and EGF receptor subfamily. ERBB4 specifically binds and it is activated by neuregulins, NRG-2, NRG-3, heparin-binding EGF-like growth factor, betacellulin, and NTAK. Interaction with these factors induces cell differentiation. ERBB4 is not activated by EGF, TGF-A, and amphiregulin. ERBB4 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody recognizes the C-term of ERBB4. Alternatively spliced variants that encode different protein isoforms with a calculated molecular weight of 144-147 kDa have been described. The apparent molecular weight of full-length ERBB4 is 150-200 kDa due to glycosylation and phosphorylation. Full-length ERBB4 can be cleaved into an 80-kDa ERBB4 intracellular fragment and a 120-kDa extracellular fragment (PMID: 11343971; 16251361).

Publications

Species	Application	Title
mouse	IF	Adv Sci (Weinh); Neuregulin4 Acts on Hypothalamic ErBb4 to Excite Oxytocin Neurons and Preserve Metabolic Homeostasis; Authors - Yi Zhang
human	IHC	Respir Res; Identification of targetable kinases in idiopathic pulmonary fibrosis.; Authors - Hisao Higo
human	WB	Front Oncol; DNAJC12 as a Mediator Between ESR1 and ERBB4 in Breast Carcinoma Cells.; Authors - Mianjie Lin
pig	WB	J Neuroinflammation; EGFR transactivation contributes to neuroinflammation in Streptococcus suis meningitis.; Authors - Xiao-Pei Yang
human	WB,IF	Cell Mol Neurobiol; Identifying Genes that Affect Differentiation of Human Neural Stem Cells and Myelination of Mature Oligodendrocytes; Authors - Dou Ye
mouse	WB	DNA Cell Biol; AK003290 Protects Myocardial Cells Against Apoptosis and Promotes Cardiac Function Recovery Via miR-539-3p/ErbB4 Axis in Ischemic-Reperfusion Injury.; Authors - Yong Tang

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