ENT1 Polyklonaler Antikörper

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• KD/KO Validated

ENT1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Rezensionen (1)

Kat-Nr. : 11337-1-AP

Synonyme

ENT1, SLC29A1

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 11337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11337-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11337-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11337-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11337-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11337-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11337-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11337-1-AP

  IP result of anti-ENT1 (IP:11337-1-AP, 3ug; Detection:11337-1-AP 1:400) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human breast cancer using 11337-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11337-1-AP (ENT1 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe, Maushodengewebe, Mauslebergewebe, Mauslungengewebe, Mausnierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 13 publications below
• IF: See 1 publications below

Produktinformation

11337-1-AP bindet in WB, IP, IF, IHC, ELISA ENT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENT1 fusion protein Ag1881
• Vollständiger Name: solute carrier family 29 (nucleoside transporters), member 1
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC008954
• Gene symbol: ENT1
• Gene ID (NCBI): 2030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENT1 (equilibrative nucleoside transporter 1; also known as SLC29A1) is a ubiquitous protein located at the cell membrane and is a major transporter responsible for the cellular uptake and release of endogenous nucleosides such as adenosine, and nucleoside analogs used in chemotherapy. The predicted molecular weight of ENT1 is 50 kDa, while heavier 54-62 kDa band may be observed due to the glycosylation (16448802, 11850433, 16111480). A smaller novel splice variant of the mouse ENT1 has also been identified, which generates a protein of 35-40 kDa (18413666).

Publikationen

Species	Application	Title
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa ; KD Validated
human	WB	Gastroenterology; ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells.; Authors - Mingyang Liu
human	WB,IHC	Theranostics; The failure of DAC to induce OCT2 expression and its remission by hemoglobin-based nanocarriers under hypoxia in renal cell carcinoma.; Authors - Lu Chen ; KD Validated
human	WB,IHC	Oncogene; Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.; Authors - Qingcai Meng
mouse	WB	Br J Pharmacol; PMID: 31166004; Authors - Zhiyuan Ma
human	WB	Am J Cancer Res; PMID: 32368394; Authors - Zeng Ye

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Parima (Verified Customer) (05-09-2023)

Since we received it in January, I have been trying to troubleshoot our western as there were so many non-specific bands so it was hard to tell which bands exactly are ENT1 bands. I have probed for ENT1 using this antibody in pancreatic cancer cell lines (BxPC3, MIA PaCa-2 and Panc-1) as well as in MDA-MB-231 breast cancer cell lines and the results were not good. I have been doing western for several years and this is the first antibody that I have a hard time troubleshooting to get nice clean bands and I even probed for ENT1 first. Can you please check if this specific lot has a problem? We had to wait for this product for so long because it was backordered and it's very disappointed to get the results we got.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 - 1:2500
• Cell Tissue Type: BxPC3, PANC-1, MIA PaCa-2, MDA-MB-231

ENT1 Antibody Western Blot validation (1:1000 - 1:2500 dilution) in BxPC3, PANC-1, MIA PaCa-2, MDA-MB-231 (Cat no:11337-1-AP)

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