DCK Polyclonal antibody

• Featured Product
• KD/KO Validated

DCK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17758-1-AP

Synonyms

DCK, deoxycytidine kinase

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Validation Data Gallery

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  WB analysis using 17758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17758-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17758-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17758-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17758-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, Jurkat cells, MCF-7 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below

Product Information

17758-1-AP targets DCK in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCK fusion protein Ag12123
• Full Name: deoxycytidine kinase
• Calculated Molecular Weight: 260 aa, 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC103764
• Gene Symbol: DCK
• Gene ID (NCBI): 1633
• RRID: AB_2261476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).

Publications

Species	Application	Title
human	WB	Oncogene; KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer.; Authors - Yaoyi Xiong
human	WB	Am J Cancer Res; PMID: 32368394; Authors - Zeng Ye
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai
human	WB	Front Oncol; m6A Methyltransferase METTL14-Mediated Upregulation of Cytidine Deaminase Promoting Gemcitabine Resistance in Pancreatic Cancer.; Authors - Congjun Zhang
human	WB	Invest New Drugs; Drug-repositioning screening identified fludarabine and risedronic acid as potential therapeutic compounds for malignant pleural mesothelioma.; Authors - Irene Dell'Anno
human	WB	Sci Rep; Using RNA-seq and targeted nucleases to identify mechanisms of drug resistance in acute myeloid leukemia.; Authors - Susan K Rathe ; KO Validated