ENO1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ENO1 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

66

Rezensionen (2)

Kat-Nr. : 11204-1-AP

Synonyme

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

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Galerie der Validierungsdaten

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  WB analysis using 11204-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11204-1-AP

  WB result of ENO1 antibody (11204-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HeLa cells.

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  WB analysis of human skeletal muscle using 11204-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11204-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11204-1-AP (ENO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11204-1-AP

  IP result of anti-ENO1 (IP:11204-1-AP, 4ug; Detection:11204-1-AP 1:8000) with mouse skeletal muscle tissue lysate 1280 ug.

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  IHC staining of human stomach cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11204-1-AP (ENO1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11204-1-AP (ENO1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11204-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 11204-1-AP (ENO1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11204-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ENO1 antibody (11204-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A549 using 11204-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human ENO1 (11204-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Skelettmuskelgewebe, L02-Zellen, Mauslebergewebe, Maus-Skelettmuskelgewebe, PC-3-Zellen, SGC-7901-Zellen
• Erfolgreiche IP: Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 55 publications below
• IHC: See 16 publications below
• IF: See 6 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

11204-1-AP bindet in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA ENO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ENO1 fusion protein Ag1692
• Vollständiger Name: enolase 1, (alpha)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC015641
• Gene symbol: ENO1
• Gene ID (NCBI): 2023
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells. And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation. (PMID: 19756395)

Publikationen

Species	Application	Title
human	WB	Cancer Cell; Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers; Authors - Yang Sun ; KD Validated
mouse	WB	Nat Neurosci; Metabolic lactate production coordinates vasculature development and progenitor behavior in the developing mouse neocortex.; Authors - Xiaoxiang Dong
human	WB,IF,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
mouse	WB,IP	Mol Cell; Riboregulation of Enolase 1 activity controls glycolysis and embryonic stem cell differentiation.; Authors - Ina Huppertz
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang
human	WB	Nucleic Acids Res; FAX-RIC enables robust profiling of dynamic RNP complex formation in multicellular organisms in vivo.; Authors - Yongwoo Na

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kihsu (Verified Customer) (06-10-2021)

its gave good looking results in WB

• Applications: Western Blot
• Cell Tissue Type: mouse liver tissues

FH

Kristian (Verified Customer) (06-07-2021)

Enolase 1 (green) is detected in the proximal tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

ENO1 Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:11204-1-AP)

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