Endothelin 1 Polyklonaler Antikörper

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• KD/KO Validated

Endothelin 1 Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 12191-1-AP

Synonyme

EDN1, Big endothelin-1, PPET1, ET1, ET 1 Big endothelin 1

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Galerie der Validierungsdaten

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  WB analysis of NIH/3T3 using 12191-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12191-1-AP (Endothelin 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse lung using 12191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 12191-1-AP (Endothelin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 12191-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 12191-1-AP (Endothelin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HUVEC using 12191-1-AP

  1X10^6 HUVEC cells were intracellularly stained with 0.4 ug Anti-Human Endothelin 1 (12191-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, Maus-Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12191-1-AP bindet in WB, IHC, IF, FC (Intra), IP, ELISA Endothelin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Endothelin 1 fusion protein Ag2847
• Vollständiger Name: endothelin 1
• Berechnetes Molekulargewicht: 212 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC009720
• Gene symbol: Endothelin 1
• Gene ID (NCBI): 1906
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EDN1, also named as Endothelin-1, PPET1 and ET-1, belongs to the endothelin/sarafotoxin family. Endothelins are endothelium-derived vasoconstrictor peptides. Emerging basic science, and animal and human data all suggest that EDN1 is a potentially important contributor in the pathobiology of fibrosing disorders, including those that affect the lung(PMID:20055532 ).

Publikationen

Species	Application	Title
mouse	WB	Redox Biol; Exercise protects aged mice against coronary endothelial senescence via FUNDC1-dependent mitophagy; Authors - Lijie Ma
human	WB, IP	Cancer Res; Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes.; Authors - Ngoc-Han Ha
mouse	IF	Redox Biol; Blocking mitochondrial cyclophilin D ameliorates TSH-impaired defensive barrier of artery.; Authors - Xiaojing Liu
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou
human	WB	Cancer Gene Ther; Systems analysis identifies endothelin 1 axis blockade for enhancing the anti-tumor effect of multikinase inhibitor.; Authors - Chae Young Hwang ; KD Validated
human	WB	J Cell Mol Med; Macrophage-derived exosomal miR-4532 promotes endothelial cells injury by targeting SP1 and NF-κB P65 signalling activation; Authors - Peng Liu

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