CREST Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CREST Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (1)

Kat-Nr. : 12439-1-AP

Synonyme

SS18L1, SS18-like protein 1, SS18 like protein 1, LP2261, KIAA0693

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Galerie der Validierungsdaten

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  WB analysis using 12439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12439-1-AP

  WB result of CREST antibody (12439-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREST transfected HeLa cells.

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  WB analysis of COLO 320 using 12439-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12439-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12439-1-AP

  IP result of anti-CREST (IP:12439-1-AP, 4ug; Detection:12439-1-AP 1:300) with HeLa cells lysate 3000 ug.

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  IP experiment of mouse brain using 12439-1-AP

  IP result of anti-CREST (IP:12439-1-AP, 3ug; Detection:12439-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 12439-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse testis using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12439-1-AP (CREST antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREST antibody (12439-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12439-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12439-1-AP (CREST antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, COLO 320-Zellen, HeLa-Zellen, humanes Herzgewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12439-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, ELISA CREST und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, drosophila, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREST fusion protein Ag3119
• Vollständiger Name: synovial sarcoma translocation gene on chromosome 18-like 1
• Berechnetes Molekulargewicht: 396 aa, 43 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC034494
• Gene symbol: CREST
• Gene ID (NCBI): 26039
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.

Publikationen

Species	Application	Title
human, mouse	IHC,CoIP	Nat Neurosci; Exome sequencing to identify de novo mutations in sporadic ALS trios.; Authors - Chesi Alessandra A
mouse	IF	J Neurosci; Kinetic Analysis of npBAF to nBAF Switching Reveals Exchange of SS18 with CREST and Integration with Neural Developmental Pathways.; Authors - Staahl Brett T BT
human	ChIP	Cancers (Basel); Global Chromatin Changes Resulting from Single-Gene Inactivation-The Role of SMARCB1 in Malignant Rhabdoid Tumor.; Authors - Colin Kenny
rat	WB,IF	J Neurosci; CREST in the Nucleus Accumbens Core Regulates Cocaine Conditioned Place Preference, Cocaine-Seeking Behavior, and Synaptic Plasticity.; Authors - Yasaman Alaghband
Drosophila	WB	PLoS Genet; Calcium-responsive transactivator (CREST) toxicity is rescued by loss of PBP1/ATXN2 function in a novel yeast proteinopathy model and in transgenic flies.; Authors - Sangeun Park
mouse	IF	Hum Mol Genet; Dysregulation of chromatin remodelling complexes in amyotrophic lateral sclerosis.; Authors - Michael Tibshirani

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aktan (Verified Customer) (12-24-2019)

The product is tested in prostate cancer cell lines. Used at the given dilution factor, in 5% BSA in PBST, o/n at 4C. Bands were detected using Licor system and Licor near IR secondary antibodies.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Prostate cancer cell lines

CREST Antibody Western Blot, validation (1:500 dilution) in Prostate cancer cell lines (Cat no:12439-1-AP)

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