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CPLX3 Polyklonaler Antikörper

CPLX3 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16949-1-AP

Synonyme

complexin 3, Complexin III, CPLX3, CPX III, CPXIII, Nbla11589

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CPLX3 Polyclonal antibody (16949-1-AP)

    WB analysis using 16949-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    WB analysis of human brain using 16949-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CPLX3 Polyclonal antibody (16949-1-AP)

    IP experiment of mouse brain using 16949-1-AP

    IP Result of anti-CPLX3 (IP:16949-1-AP, 3ug; Detection:16949-1-AP 1:300) with mouse brain tissue lysate 6000ug.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMaushirngewebe, humanes Hirngewebe
Erfolgreiche IPMaushirngewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 1 publications below
IHCSee 1 publications below

Produktinformation

16949-1-AP bindet in WB, IP, IHC,ELISA CPLX3 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte ReaktivitätMaus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CPLX3 fusion protein Ag10571
Vollständiger Name complexin 3
Berechnetes Molekulargewicht 158 aa, 18 kDa
Beobachtetes Molekulargewicht 20-23 kDa
GenBank-ZugangsnummerBC018026
Gene symbol CPLX3
Gene ID (NCBI) 594855
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPLX3, also named as Complexin-3, is 158 amino acid protein, which belongs to the complexin/synaphin family. CPLX3 localizes in the membrane and binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. CPLX3 positively regulates a late step in synaptic vesicle exocytosis.

Protokolle

Produktspezifische Protokolle
WB protocol for CPLX3 antibody 16949-1-APProtokoll herunterladen
IP protocol for CPLX3 antibody 16949-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIHC

Proc Natl Acad Sci U S A

Extracortical origin of some murine subplate cell populations.

Authors - María Pedraza
View Article
mouseWB

Free Radic Biol Med

NOX2 Deficiency Ameliorates Cerebral Injury by Reduction of Complexin II-mediated Glutamate Excitotoxicity in Experimental Stroke.

Authors - Wang Ziying Z
View Article