COX5A Polyclonal antibody

COX5A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11448-1-AP

Synonyms

COX, COX VA, COX5A, VA



Tested Applications

Positive WB detected inmouse brain tissue, human liver tissue, rat brain tissue, L02 cells, HeLa cells
Positive IHC detected inhuman gliomas tissue, human colon tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11448-1-AP targets COX5A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey, zebrafish, pig, chimpanzee
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen COX5A fusion protein Ag2005
Full Name cytochrome c oxidase subunit Va
Calculated molecular weight 150 aa, 17 kDa
Observed molecular weight 13 kDa
GenBank accession numberBC024240
Gene symbol COX5A
Gene ID (NCBI) 9377
RRIDAB_2085429
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).

Protocols

Product Specific Protocols
WB protocol for COX5A antibody 11448-1-APDownload protocol
IHC protocol for COX5A antibody 11448-1-APDownload protocol
IF protocol for COX5A antibody 11448-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Authors - Qinghai Zhang
humanWB

Cancer Res

CK1δ/CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma

Authors - Karen L Burger
mouseWB

Dev Cell

Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain.

Authors - Shefali Krishna
humanWB

Cell Death Dis

O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.

Authors - Weicheng Wu
pigIHC

Int J Mol Sci

Relation of Plasma Catecholamine Concentrations and Myocardial Mitochondrial Respiratory Activity in Anesthetized and Mechanically Ventilated, Cardiovascular Healthy Swine

Authors - Nadja Abele
ratWB

Cell Death Dis

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Authors - Yuehong Wang