COX5A Polyclonal antibody

COX5A Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 11448-1-AP

Synonyms

COX, COX VA, COX5A, VA

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Validation Data Gallery

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  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11448-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11448-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

• 

  WB analysis of human liver using 11448-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11448-1-AP (COX5A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11448-1-AP

  Immunofluorescent analysis of HeLa cells using 11448-1-AP (COX5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human liver tissue, rat brain tissue, L02 cells, HeLa cells
• Positive IHC detected in: human gliomas tissue, human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 12 publications below
• IHC: See 2 publications below

Product Information

11448-1-AP targets COX5A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chimpanzee, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX5A fusion protein Ag2005
• Full Name: cytochrome c oxidase subunit Va
• Calculated molecular weight: 150 aa, 17 kDa
• Observed molecular weight: 13 kDa
• GenBank accession number: BC024240
• Gene symbol: COX5A
• Gene ID (NCBI): 9377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COX5A(Cytochrome c oxidase subunit 5A,mitochondrial)is also named as COXVA and belongs to the cytochrome c oxidase subunit 5A family.This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).

Publications

Species	Application	Title
zebrafish		Nucleic Acids Res; Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism.; Authors - Danni Chen
rat	WB	Chin J Integr Med; Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin.; Authors - Bin Chen
human	IHC	Hum Pathol; Diagnostic biomarkers for renal cell carcinoma: selection using novel bioinformatics systems for microarray data analysis.; Authors - Osunkoya Adeboye O AO
monkey,chimpanzee,human	IHC	BMC Evol Biol; Molecular evolution of the cytochrome c oxidase subunit 5A gene in primates.; Authors - Uddin Monica M
human	WB	Invest Ophthalmol Vis Sci; Leber's Hereditary Optic Neuropathy Arising From the Synergy Between ND1 3635G>A Mutation and Mitochondrial YARS2 Mutations.; Authors - Xiaofen Jin
rat	WB	PLoS One; PMID: 25436770; Authors - Ying-Ying Gong