COX5A Polyclonal antibody

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  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 11448-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11448-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 11448-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11448-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11448-1-AP (COX5A Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11448-1-AP (COX5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11448-1-AP (COX5A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11448-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11448-1-AP (COX5A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11448-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX5A antibody (11448-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

COX5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11448-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COX VA, COX5, COXVA

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells, rat brain tissue, SH-SY5Y cells
• Positive IHC detected in: human colon tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 20 publications below
• IHC: See 3 publications below

Product Information

11448-1-AP targets COX5A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, monkey, zebrafish, chimpanzee
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: COX5A fusion protein Ag2005
• Full Name: cytochrome c oxidase subunit Va
• Calculated Molecular Weight: 150 aa, 17 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC024240
• Gene Symbol: COX5A
• Gene ID (NCBI): 9377
• RRID: AB_2085429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20674
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COX5A(Cytochrome c oxidase subunit 5A, mitochondrial)is also named COXVA and belongs to the cytochrome c oxidase subunit 5A family. This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).

Publications

Species	Application	Title
	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
human	WB	Cancer Res; CK1δ/CK1ε Signaling Sustains Mitochondrial Metabolism and Cell Survival in Multiple Myeloma; Authors - Karen L Burger
mouse	WB	Dev Cell; Identification of long-lived proteins in the mitochondria reveals increased stability of the electron transport chain.; Authors - Shefali Krishna
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
pig	IHC	Int J Mol Sci; Relation of Plasma Catecholamine Concentrations and Myocardial Mitochondrial Respiratory Activity in Anesthetized and Mechanically Ventilated, Cardiovascular Healthy Swine; Authors - Nadja Abele
rat	WB	Cell Death Dis; Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.; Authors - Yuehong Wang