COX5A Polyclonal antibody
COX5A Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11448-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human liver tissue, rat brain tissue, L02 cells, HeLa cells |
Positive IHC detected in | human gliomas tissue, human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 12 publications below |
IHC | See 2 publications below |
Product Information
11448-1-AP targets COX5A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chimpanzee, monkey, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | COX5A fusion protein Ag2005 |
Full Name | cytochrome c oxidase subunit Va |
Calculated molecular weight | 150 aa, 17 kDa |
Observed molecular weight | 13 kDa |
GenBank accession number | BC024240 |
Gene symbol | COX5A |
Gene ID (NCBI) | 9377 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
COX5A(Cytochrome c oxidase subunit 5A,mitochondrial)is also named as COXVA and belongs to the cytochrome c oxidase subunit 5A family.This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.The mature human COX Va polypeptide shares 35% and 95% protein sequence identity with the corresponding S. cerevisiae COX subunit VI and bovine COX Va, respectively(PMID:2853101).
Protocols
Product Specific Protocols | |
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WB protocol for COX5A antibody 11448-1-AP | Download protocol |
IHC protocol for COX5A antibody 11448-1-AP | Download protocol |
IF protocol for COX5A antibody 11448-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res Deletion of Gtpbp3 in zebrafish revealed the hypertrophic cardiomyopathy manifested by aberrant mitochondrial tRNA metabolism. | ||
Chin J Integr Med Electroacupuncture Attenuates Ischemic Brain Injury and Cellular Apoptosis via Mitochondrial Translocation of Cofilin. | ||
Hum Pathol Diagnostic biomarkers for renal cell carcinoma: selection using novel bioinformatics systems for microarray data analysis. | ||
BMC Evol Biol Molecular evolution of the cytochrome c oxidase subunit 5A gene in primates. | ||
Invest Ophthalmol Vis Sci Leber's Hereditary Optic Neuropathy Arising From the Synergy Between ND1 3635G>A Mutation and Mitochondrial YARS2 Mutations. | ||