CD47 Monoklonaler Antikörper

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• KD/KO Validated

CD47 Monoklonal Antikörper für FC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

1E1D8

Kat-Nr. : 66304-1-Ig

Synonyme

CD47, CD47 molecule, IAP, Integrin associated protein, Leukocyte surface antigen CD47, MER6, OA3, Protein MER6

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Galerie der Validierungsdaten

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  WB analysis of A549 using 66304-1-Ig

  WB result of CD47 antibody (66304-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD47 transfected A549 cells.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66304-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66304-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 66304-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 66304-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66304-1-Ig (CD47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HUVEC using 66304-1-Ig

  1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human CD47 (66304-1-Ig, Clone:1E1D8) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen, Jurkat-Zellen, K-562-Zellen, NCI-H1299-Zellen, SKOV-3-Zellen, TF-1-Zellen
• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

66304-1-Ig bindet in WB, IF, FC, ELISA CD47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD47 fusion protein Ag14132
• Vollständiger Name: CD47 molecule
• Berechnetes Molekulargewicht: 323 aa, 35 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC010016
• Gene symbol: CD47
• Gene ID (NCBI): 961
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

Publikationen

Species	Application	Title
human,mouse	WB	J Control Release; Erythrocyte-biomimetic nanosystems to improve antitumor effects of paclitaxel on epithelial cancers.; Authors - Mingming Song
mouse	WB	J Nanobiotechnology; Bone-targeted erythrocyte-cancer hybrid membrane-camouflaged nanoparticles for enhancing photothermal and hypoxia-activated chemotherapy of bone invasion by OSCC.; Authors - Hongying Chen
human	WB	RSC Adv; Seedless preparation of Au nanorods by hydroquinone assistant and red blood cell membrane camouflage; Authors - Jing Li
human	IF,FC	J Cell Commun Signal; CD47 interactions with exportin-1 limit the targeting of m 7 G-modified RNAs to extracellular vesicles; Authors - Sukhbir Kaur
human	WB	J Cancer Res Clin Oncol; B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy; Authors - Siji Chen
human	WB	J Clin Lab Anal; MicroRNA-148a-3p inhibits cancer progression and is a novel screening biomarker for gastric cancer.; Authors - Chenhui Bao

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