CD3 Delta Polyklonaler Antikörper

CD3 Delta Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 16669-1-AP

Synonyme

CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D, TCRδ

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Galerie der Validierungsdaten

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  WB analysis using 16669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16669-1-AP (CD3 delta antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16669-1-AP

  IP Result of anti-CD3 delta (IP:16669-1-AP, 4ug; Detection:16669-1-AP 1:800) with Jurkat cells lysate 2800ug.

• 

  IF Staining of human tonsillitis using 16669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 Delta antibody (16669-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 16669-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 Delta antibody (16669-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 16669-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD3 delta antibody (16669-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Maus-Thymusgewebe, MOLT-4-Zellen, Ratten-Thymusgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

16669-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CD3 Delta und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD3 Delta fusion protein Ag10148
• Vollständiger Name: CD3d molecule, delta (CD3-TCR complex)
• Berechnetes Molekulargewicht: 171 aa, 19 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC070321
• Gene symbol: CD3D
• Gene ID (NCBI): 915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publikationen

Species	Application	Title
mouse	IHC	Cancer Res; Epigenetic regulation of CXCL12 plays a critical role in mediating tumor progression and the immune response in osteosarcoma.; Authors - Binghao Li
		Clin Cancer Res; Changes in Expression of Genes Representing Key Biologic Processes after Neoadjuvant Chemotherapy in Breast Cancer, and Prognostic Implications in Residual Disease.; Authors - Marie Klintman
mouse	IF	Inflamm Regen; Testosterone upregulates glial cell line-derived neurotrophic factor (GDNF) and promotes neuroinflammation to enhance glioma cell survival and proliferation; Authors - Kouminin Kanwore
human	IHC	Ann Diagn Pathol; Diagnostic utility of the aberrant immunohistochemical expression of CD3 molecules for peripheral T-cell lymphomas.; Authors - Tsugumi Satoh
mouse	WB,IP	Cell Rep; cis interaction of CD153 with TCR/CD3 is crucial for the pathogenic activation of senescence-associated T cells; Authors - Yuji Fukushima
mouse	IF	Cell Discov; Single-cell transcriptomics reveals intestinal cell heterogeneity and identifies Ep300 as a potential therapeutic target in mice with acute liver failure; Authors - Jie Yin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anja (Verified Customer) (12-22-2023)

The antibody was tested for western blot but only showed unspecific results. Also comparison to validated negative controls did not indicate any specificity in our hands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Thymocytes and T cells

CD3 Delta Antibody Western Blot validation (1:1000 dilution) in Thymocytes and T cells (Cat no:16669-1-AP)

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