CD24 Polyklonaler Antikörper

CD24 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 10600-1-AP

Synonyme

CD24, CD24 molecule, CD24A, Signal transducer CD24

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Galerie der Validierungsdaten

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  WB analysis using 10600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 10600-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10600-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10600-1-AP (CD24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human appendicitis using 10600-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendicitis using 10600-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 10600-1-AP (CD24 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, Jurkat-Zellen, Maushirngewebe, Raji-Zellen, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Appendizitis-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

10600-1-AP bindet in WB, IF, ELISA CD24 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD24 fusion protein Ag0654
• Vollständiger Name: CD24 molecule
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 30-70 kDa
• GenBank-Zugangsnummer: BC007674
• Gene symbol: CD24
• Gene ID (NCBI): 100133941
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Publikationen

Species	Application	Title
rat		J Neuroinflammation; Coding transcriptome analyses reveal altered functions underlying immunotolerance of PEG-fused rat sciatic nerve allografts.; Authors - Tyler A Smith
human	WB	Int J Mol Sci; Preclinical Repurposing of Sitagliptin as a Drug Candidate for Colorectal Cancer by Targeting CD24/CTNNB1/SOX4-Centered Signaling Hub; Authors - Jing-Wen Shih
human		Thyroid; Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.; Authors - Kai Guo
rat	WB	Stem Cells Int; Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions.; Authors - Arjun Sinkemani
human		Adv Sci (Weinh); A Novel Long Non-Coding RNA lnc030 Maintains Breast Cancer Stem Cell Stemness by Stabilizing SQLE mRNA and Increasing Cholesterol Synthesis.; Authors - Yilu Qin
human	WB	Int J Biol Sci; VEGFA/NRP-1/GAPVD1 axis promotes progression and cancer stemness of triple-negative breast cancer by enhancing tumor cell-macrophage crosstalk; Authors - Lu Wang

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