Tested Applications
| Positive WB detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human tonsillitis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60208-2-Ig targets CD23 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0425 Product name: Recombinant human CD23,FCER2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 48-248 aa of BC064417 Sequence: DTTQSLKQLEERAARNVSQVSKNLESHHGDQMAQKSQSTQISQELEELRAEQQRLKSQDLELSWNLNGLQADLSSFKSQELNERNEASDLLERLREEVTKLRMELQVSSGFVCNTCPEKWINFQRKCYYFGKGTKQWVHARYACDDMEGQLVSIHSPEEQDFLTKHASHTGSWIGLRNLDLKGEFIWVDGSHVDYSNWAPG Predict reactive species |
| Full Name | Fc fragment of IgE, low affinity II, receptor for (CD23) |
| Calculated Molecular Weight | 321 aa, 36 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC064417 |
| Gene Symbol | CD23 |
| Gene ID (NCBI) | 2208 |
| RRID | AB_11232221 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P06734 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD23 antibody 60208-2-Ig | Download protocol |
| IHC protocol for CD23 antibody 60208-2-Ig | Download protocol |
| WB protocol for CD23 antibody 60208-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD23 Monoclonal antibody (4C7C9), catalog number 60208-2-Ig, has 2 published citations. These appear in Clinical and Translational Medicine (2023) and Clinical and Translational Oncology (2025). The research fields encompass oncology, specifically breast cancer prognosis and neoadjuvant therapy response prediction, as well as colorectal cancer microsatellite status and lymph node metastasis analysis. The cited application is immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Clin Transl Med Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy | ||
Clin Transl Oncol The influence of microsatellite status on tertiary lymphoid structures and lymph node metastasis in colorectal cancer |
