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CD2 Polyklonaler Antikörper

CD2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10299-1-AP

Synonyme

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of various lysates using CD2 Polyclonal antibody (10299-1-AP)

    WB analysis using 10299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10299-1-AP (CD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Polyclonal antibody (10299-1-AP)

    IHC staining of human tonsillitis using 10299-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD2 Polyclonal antibody (10299-1-AP)

    IHC staining of human tonsillitis using 10299-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen
Erfolgreiche Detektion in IHChumanes Tonsillitisgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunhistochemie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

10299-1-AP bindet in WB, IHC, ELISA CD2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD2 fusion protein Ag0423
Vollständiger Name CD2 molecule
Berechnetes Molekulargewicht 351 aa, 39 kDa
Beobachtetes Molekulargewicht 50-60 kDa
GenBank-ZugangsnummerBC033583
Gene symbol CD2
Gene ID (NCBI) 914
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protokolle

Produktspezifische Protokolle
WB protocol for CD2 antibody 10299-1-APProtokoll herunterladen
IHC protocol for CD2 antibody 10299-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other