CD107a / LAMP1 Polyklonaler Antikörper

CD107a / LAMP1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

95

Rezensionen (3)

Kat-Nr. : 21997-1-AP

Synonyme

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker

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Galerie der Validierungsdaten

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  WB analysis using 21997-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 21997-1-AP (LAMP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21997-1-AP (CD107a / LAMP1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21997-1-AP (LAMP1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 21997-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 21997-1-AP (LAMP1 antibody at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, U937 cells
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Gliomgewebe, humanes Pankreasgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 64 publications below
• IHC: See 5 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Produktinformation

21997-1-AP bindet in WB, IP, IHC, FC, ELISA CD107a / LAMP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, Rind, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD107a / LAMP1 fusion protein Ag17367
• Vollständiger Name: lysosomal-associated membrane protein 1
• Berechnetes Molekulargewicht: 417 aa, 45 kDa
• Beobachtetes Molekulargewicht: 90-120 kDa
• GenBank-Zugangsnummer: BC021288
• Gene symbol: LAMP1
• Gene ID (NCBI): 3916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Publikationen

Species	Application	Title
		Immunity; The Endotoxin Delivery Protein HMGB1 Mediates Caspase-11-Dependent Lethality in Sepsis.; Authors - Meihong Deng
human		Adv Sci (Weinh); Inhibition of CK2/ING4 Pathway Facilitates Non-Small Cell Lung Cancer Immunotherapy; Authors - Qian Gou
	WB	Arthritis Rheumatol; LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands; Authors - Hiroyuki Nakamura
human		Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang
human		Nat Commun; Mismatch repair deficiency predicts response to HER2 blockade in HER2-negative breast cancer.; Authors - Nindo B Punturi
human	WB	Sci Total Environ; TFEB coordinates autophagy and pyroptosis as hepatotoxicity responses to ZnO nanoparticles; Authors - Xingyao Pei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Marina (Verified Customer) (08-08-2022)

Western blot against LAMP1 in human breast cancer cell line HCC1937. 1:1000 dilution, overnight incubation at 4ºC. A clear band can be observed between 95 and 130 kDa. Ponceau S staining is included as total protein loading control.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human breast cancer cells

CD107a / LAMP1 Antibody Western Blot validation (1:1000 dilution) in Human breast cancer cells (Cat no:21997-1-AP)

FH

Azita (Verified Customer) (06-17-2021)

Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by LAMP1 Polyclonal antibody at dilution of 1:50 showed strong labelling.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NSC-34 cells

FH

Mayru (Verified Customer) (04-30-2019)

The antibody works great for detection of LAMP1 protein expression using Western blot analysis.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H4 cells

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