LAMP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

LAMP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 12632-1-AP

Synonyms

CD208, LAMP 3, LAMP, DC-lysosome-associated membrane glycoprotein, DCLAMP

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Validation Data Gallery

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  WB analysis using 12632-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 12632-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12632-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12632-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12632-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12632-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12632-1-AP (LAMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12632-1-AP

  IP result of anti-LAMP3 (IP:12632-1-AP, 4ug; Detection:12632-1-AP 1:500) with A549 cells lysate 2800ug.

• 

  IHC staining of human lung using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12632-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12632-1-AP (LAMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 12632-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAMP3 antibody (12632-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A375 cells, mouse thymus tissue, rat lung tissue, A549 cells, mouse lung tissue, HEK-293T cells, Raji cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung tissue, human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 10 publications below

Product Information

12632-1-AP targets LAMP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAMP3 fusion protein Ag3325
• Full Name: lysosomal-associated membrane protein 3
• Calculated Molecular Weight: 416 aa, 44 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC032940
• Gene Symbol: LAMP3
• Gene ID (NCBI): 27074
• RRID: AB_2076629
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).

Publications

Species	Application	Title
human	WB	Nat Med; Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.; Authors - Yingxiao Shi
human	IF	Arthritis Rheumatol; LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands; Authors - Hiroyuki Nakamura
monkey	WB	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
human	WB,IF	J Clin Invest; Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.; Authors - Ying-Qian Mo
human	WB,IF	Autophagy; LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.; Authors - Tsutomu Tanaka
mouse	IF	Cell Prolif; Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.; Authors - Xiaofan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ying (Verified Customer) (08-19-2019)

very good antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse lung tissues

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