LAMP3 Polyclonal antibody

LAMP3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12632-1-AP

Synonyms

CD208, DC LAMP, DCLAMP, LAMP, LAMP 3, LAMP3, Protein TSC403, TSC403



Tested Applications

Positive WB detected inA375 cells, mouse thymus tissue, rat lung tissue, A549 cells, mouse lung tissue, HEK-293T cells, Raji cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12632-1-AP targets LAMP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LAMP3 fusion protein Ag3325
Full Name lysosomal-associated membrane protein 3
Calculated Molecular Weight 416 aa, 44 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC032940
Gene Symbol LAMP3
Gene ID (NCBI) 27074
RRIDAB_2076629
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).

Protocols

Product Specific Protocols
WB protocol for LAMP3 antibody 12632-1-APDownload protocol
IHC protocol for LAMP3 antibody 12632-1-APDownload protocol
IF protocol for LAMP3 antibody 12632-1-APDownload protocol
IP protocol for LAMP3 antibody 12632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Med

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Authors - Yingxiao Shi
humanIF

Arthritis Rheumatol

LAMP3 induces lysosome-dependent cell death by impairing autophagic caspase-8 degradation in Sjögren's salivary glands

Authors - Hiroyuki Nakamura
monkeyWB

Nat Commun

Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.

Authors - Xianding Sun
humanWB,IF

J Clin Invest

Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.

Authors - Ying-Qian Mo
humanWB,IF

Autophagy

LAMP3 inhibits autophagy and contributes to cell death by lysosomal membrane permeabilization.

Authors - Tsutomu Tanaka
mouseIF

Cell Prolif

Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease.

Authors - Xiaofan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ying (Verified Customer) (08-19-2019)

very good antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse lung tissues