Catalase Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Catalase Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (6)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

128

Rezensionen (4)

Kat-Nr. : 21260-1-AP

Synonyme

CAT, catalase, Cell and organelle markers, Peroxisome Marker

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Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 21260-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21260-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21260-1-AP

  WB result of Catalase antibody (21260-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Catalase transfected HeLa cells.

• 

  WB analysis of rat liver using 21260-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 21260-1-AP (CAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21260-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21260-1-AP (Catalase Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21260-1-AP (Catalase antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 21260-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 21260-1-AP (CAT antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21260-1-AP

  Immunofluorescent analysis of HepG2 cells using 21260-1-AP (Catalase antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of HeLa using 21260-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Catalase antibody (21260-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, L02-Zellen, Mauslebergewebe, Raji-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 121 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• FC: See 1 publications below

Produktinformation

21260-1-AP bindet in WB, IHC, IF, FC, ELISA Catalase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind, Ziege, Meretrix meretrix, Pilz, Fledermaus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Catalase fusion protein Ag15383
• Vollständiger Name: catalase
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC112219
• Gene symbol: CAT
• Gene ID (NCBI): 847
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
mouse	WB	Nat Commun; Senescence-associated 13-HODE production promotes age-related liver steatosis by directly inhibiting catalase activity; Authors - Jinjie Duan
mouse	WB	Nat Commun; Programmable probiotics modulate inflammation and gut microbiota for inflammatory bowel disease treatment after effective oral delivery.; Authors - Jun Zhou
mouse	WB	Nat Commun; The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.; Authors - Guang Yang
human	WB	Redox Biol; LRPPRC regulates redox homeostasis via the circANKHD1/FOXM1 axis to enhance bladder urothelial carcinoma tumorigenesis.; Authors - Wen-Su Wei
mouse	WB	Cell Rep; The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.; Authors - Wu Hongtan H

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Silvia (Verified Customer) (08-11-2022)

It worked well for western blotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human fibroblasts

FH

Maximilian (Verified Customer) (06-03-2022)

The antibody works fine and detected human catalase at 0.01 and 0.05 µg total protein amount in my blot. Accidental storage time of 24h at RT did not affect performance.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2

FH

Maximilian (Verified Customer) (05-27-2022)

Antibody performed well in detection of catalase standard protein (catalase from human erythrocytes) but signal for cell lysates could be better (will increase antibody concentration). Maybe this is due to the storage for 24h at room temperature, which was the result of wrong storage by a colleague, as the package was erroneously delivered to her instead of me.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HepG2

FH

Tom (Verified Customer) (08-25-2021)

Membrane blocked in BSA solution prior to incubation with antibody (1:2000). Correct band is clearly visible, but with some background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

Catalase Antibody Western Blot validation (1:2000 dilution) in (Cat no:21260-1-AP)

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