SOD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SOD3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14316-1-AP

Synonyms

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3

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Validation Data Gallery

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  WB analysis using 14316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14316-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14316-1-AP (SOD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 14316-1-AP

  IP result of anti-SOD3 (IP:14316-1-AP, 4ug; Detection:14316-1-AP 1:1000) with human placenta tissue lysate 2800ug.

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Tested Applications

• Positive WB detected in: human urine sample, human heart tissue, human urine, human placenta tissue
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below

Product Information

14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SOD3 fusion protein Ag5556
• Full Name: superoxide dismutase 3, extracellular
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25-33 kDa
• GenBank Accession Number: BC014418
• Gene Symbol: SOD3
• Gene ID (NCBI): 6649
• RRID: AB_10898170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2. SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

Publications

Species	Application	Title
mouse,human	WB	Br J Pharmacol; Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.; Authors - Ai Wei
mouse	WB	J Proteome Res; Sex-Specific Alterations in Placental Proteomics Induced by Intrauterine Hyperglycemia; Authors - Zhuo-Ran Ren
human	WB	Transl Pediatr; Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.; Authors - Gedong Ming
human,rat	WB	Toxicol Appl Pharmacol; (-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.; Authors - Xue Tian
human	WB	Clin Immunol; Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma; Authors - Xisong Liang ; KD Validated
mouse	WB	Am J Transl Res; Artemether regulates liver glycogen and lipid utilization through mitochondrial pyruvate oxidation in db/db mice; Authors - Wenci Weng