C9orf72 Monoklonaler Antikörper

C9orf72 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

3D2H6

Kat-Nr. : 66140-1-Ig

Synonyme

C9RANT

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Galerie der Validierungsdaten

• 

  WB analysis of human brain using 66140-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 66140-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66140-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66140-1-Ig (C9orf72 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of C6 using 66140-1-Ig

  IP Result of anti-C9orf72 (IP:66140-1-Ig, 4ug; Detection:66140-1-Ig 1:1000) with C6 cells lysate 1320ug.

• 

  IHC staining of human gliomas using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66140-1-Ig (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66140-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain using 66140-1-Ig(C9orf72 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 66140-1-Ig

  Immunofluorescent analysis of SH-SY5Y cells using 66140-1-Ig (C9orf72 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, C6-Zellen, Neuro-2a-Zellen
• Erfolgreiche IP: C6-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

66140-1-Ig bindet in WB, IP, IHC, IF, ELISA C9orf72 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: C9orf72 fusion protein Ag21080
• Vollständiger Name: chromosome 9 open reading frame 72
• Berechnetes Molekulargewicht: 481 aa, 54 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC020851
• Gene symbol: C9orf72
• Gene ID (NCBI): 203228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). FTLD-TDP has five subtypes: Sporadic FTLD, GRN mutation FTLD, TARDBP mutation FTLD, VCP mutation FTLD and C9ORF72 mutation FTLD. The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa.

Publikationen

Species	Application	Title
human,mouse	WB,IF	Acta Neuropathol Commun; Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.; Authors - Petra Frick
human	WB	Sci Rep; Dual-gRNA approach with limited off-target effect corrects C9ORF72 repeat expansion in vivo.; Authors - Xuejiao Piao
human	IHC	Amyotroph Lateral Scler Frontotemporal Degener; Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.; Authors - Yvonne S Davidson
human	IHC	Acta Neuropathol; Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.; Authors - Shahram Saberi
rat	WB	Animal Model Exp Med; Knock in of a hexanucleotide repeat expansion in the C9orf72 gene induces ALS in rats.; Authors - Wei Dong
human	WB	Nat Neurosci; C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.; Authors - Rajeeve Sivadasan

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