C9orf72 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

C9orf72 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 25757-1-AP

Synonyme

C9orf72, C9RANT

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 25757-1-AP

  WB result of C9orf72 antibody (25757-1-AP, 1:250 incubated at RT for 1 hour) with si-C9orf72 HEK293 cells, C9orf72 CRISPR KO HEK293 cells; normal HEK293 cells and non-targeting control (NTC) siRNA transfected HEK293 cells as control. The ~50 kDa is the C9orf72 Long isoform. (Data from Dr Chris Webster, Postdoc in Kurt de Vos’s group at SITRAN).

• 

  WB analysis of mouse brain using 25757-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25757-1-AP (C9orf72 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25757-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25757-1-AP (C9orf72 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human gliomas using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25757-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 25757-1-AP (C9orf72 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25757-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25757-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25757-1-AP (C9orf72 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse brain using 25757-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 25757-1-AP (C9orf72 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1500
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

25757-1-AP bindet in WB, IHC, IF, ELISA C9orf72 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: C9orf72 fusion protein Ag22723
• Vollständiger Name: chromosome 9 open reading frame 72
• Berechnetes Molekulargewicht: 481 aa, 54 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC068445
• Gene symbol: C9orf72
• Gene ID (NCBI): 203228
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

C9ORF72 has a domain whith polymorphic hexanucleotide repeat (GGGGCC). The C9ORF72-hexanucleotide repeat expansions have been recently identified as genetic markers in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). The C9ORF72 repeat expansions may indicate a worse prognosis in ALS. Human C9ORF72 has some isoforms with MW 54-60 kDa and 25-30 kDa. Mouse C9orf72 has some isoforms with MW 50-60 kDa and 35 kDa. This antibody detects the N-terminal of C9orf72.

Publikationen

Species	Application	Title
human	IHC	Nat Med; Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.; Authors - Yingxiao Shi
human	WB	Acta Neuropathol; An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.; Authors - Claudia S Bauer
human	WB,IF	Elife; Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.; Authors - Carl Laflamme
human,rat	WB	Theranostics; C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α; Authors - Wenzhong Zheng
human	WB	Front Cell Neurosci; Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation; Authors - Claudia S Bauer
human	WB,IF	EMBO Rep; C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains; Authors - Julia Jülg ; KO Validated

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