Beta Actin Monoklonaler Antikörper

Beta Actin Monoklonal Antikörper für WB

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human, Maus, Pflanze, Ratte, Zebrafisch und mehr (4)

Anwendung

WB, IF, ELISA

Konjugation

HRP Fluorescent Dye

Publikationen

263

CloneNo.

7D2C10

Rezensionen (7)

Kat-Nr. : HRP-60008

Synonyme

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, Cell and organelle markers, Cytoskeleton Marker, F actin, HRP β actin, HRP-ACTB, HRP-Beta actin, HRP-F actin, PS1TP5BP1, β actin

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Galerie der Validierungsdaten

• 

  WB analysis of multi-cells/tissue using HRP-60008

  Western blot analysis of beta actin in various tissues and cell lines using Proteintech antibody HRP-60008 at a dilution of 1:5000.

• 

  WB analysis of HeLa using HRP-60008

  HeLa cells were subjected to SDS PAGE followed by western blot with HRP-60008 (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 253 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Produktinformation

HRP-60008 bindet in WB, IF, ELISA Beta Actin und zeigt Reaktivität mit human, Maus, Pflanze, Ratten, Zebrafisch

• Getestete Reaktivität: human, Maus, Pflanze, Ratte, Zebrafisch
• In Publikationen genannte Reaktivität: human, Affe, hamster, Huhn, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: actin, beta
• Berechnetes Molekulargewicht: 375 aa, 42 kDa
• Beobachtetes Molekulargewicht: 42 kDa
• GenBank-Zugangsnummer: BC002409
• Gene symbol: ACTB
• Gene ID (NCBI): 60
• Konjugation: HRP Fluorescent Dye
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publikationen

Species	Application	Title
	WB	Cell; Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate; Authors - Kazushige Shiraishi
	WB	Nat Neurosci; Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.; Authors - Kornélia Szebényi
mouse	WB	Blood; Fetal liver hepcidin secures iron stores in utero.; Authors - Lara Kämmerer
human	WB	Nat Microbiol; Adeno-associated virus 2 bound to its cellular receptor AAVR.; Authors - Ran Zhang
human	WB	Mol Cancer; CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer.; Authors - Ya Fan
human	WB	Nat Chem Biol; Identification of a cellularly active SIRT6 allosteric activator.; Authors - Zhimin Huang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (04-20-2023)

The beta actin works very well diluted at 1:5000. It was used on T-ALL cell lines. Loading 30 ug of whole protein lysate.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: T cells

FH

Priya (Verified Customer) (04-17-2023)

Used for Caco2 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 5000
• Cell Tissue Type: Caco2, Mice colon

FH

Iru (Verified Customer) (06-07-2021)

The antibody works well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

FH

Azita (Verified Customer) (06-02-2021)

Western blot analysis using HRP- conjugated beta actin in NSC34 cell line at dilution of 1:10000

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: NSC34 cells

HRP Beta Actin Antibody Western Blot validation (1:10000 dilution) in NSC34 cells (Cat no:HRP-60008)

FH

Hasibur (Verified Customer) (08-01-2019)

Nice antibody and no need for secondary antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:20000
• Cell Tissue Type: Bladder Cancer

FH

Joanne (Verified Customer) (01-22-2019)

Tested using 10ug of Caco-2 cell lysate and whole membrane. A strong band with no background and a great time saver!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: caco-2 cell

HRP Beta Actin Antibody Western Blot, validation (1:5000 dilution) in caco-2 cell (Cat no:HRP-60008)

FH

Tess (Verified Customer) (11-29-2018)

Little to no background, and takes half the time.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: human colorectal cancer cell line

HRP Beta Actin Antibody Western Blot, validation (1:10,000 dilution) in human colorectal cancer cell line (Cat no:HRP-60008)

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