Beta Actin Polyclonal antibody

• Featured Product
• KD/KO Validated

Beta Actin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine, monkey and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

3881

Reviews (7)

Cat no : 20536-1-AP

Synonyms

Actin, ACTB, bate actin, β actin, Beta-actin

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Validation Data Gallery

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  WB analysis using 20536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 cells using 20536-1-AP

  A549 cells (shcontrol and shRNA of Beta actin) were subjected to SDS PAGE followed by western blot with 20536-1-AP (ACTB antibody) at dilution of 1:500.

• 

  WB analysis using 20536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (Beta Actin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 20536-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta actin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20536-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20536-1-AP (beta Actin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 20536-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20536-1-AP (Beta Actin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20536-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 20536-1-AP (Beta Actin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20536-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20536-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20536-1-AP (beta actin Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MDCK using 20536-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Beta Actin antibody (20536-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, C6 cells, mouse colon tissue, Caco-2 cells, RAW 264.7 cells, SMMC-7721 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain tissue, rat brain tissue, NIH/3T3 cells, mouse liver tissue, rat kidney tissue, rat spleen tissue, rat liver tissue
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells

Recommended dilution

• Western Blot (WB): WB : 1:4000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3774 publications below
• IHC: See 4 publications below
• IF: See 14 publications below
• IP: See 7 publications below
• ELISA: See 1 publications below
• CoIP: See 2 publications below

Product Information

20536-1-AP targets Beta Actin in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat, canine, monkey samples.

• Tested Reactivity: human, mouse, rat, canine, monkey
• Cited Reactivity: human, mouse, rat, chicken, goat, sheep, yeast, ochotona curzoniae, camelus bactrianus, carp
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Actin fusion protein Ag14521
• Full Name: actin, beta
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC002409
• Gene Symbol: Beta Actin
• Gene ID (NCBI): 60
• RRID: AB_10700003
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	WB	Nature; Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size.; Authors - Matthew B Johnson
	WB	Cell; Microglia jointly degrade fibrillar alpha-synuclein cargo by distribution through tunneling nanotubes.; Authors - Hannah Scheiblich
	WB	Cell Discov; Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability; Authors - Xinyu Zhang
mouse	WB	Adv Mater; Manganese@Albumin Nanocomplex and Its Assembled Nanowire Activate TLR4-Dependent Signaling Cascades of Macrophages; Authors - Shuodan Huang
	WB	Cell Mol Immunol; cFLIPS regulates alternative NLRP3 inflammasome activation in human monocytes; Authors - Yuhui Gao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Julia (Verified Customer) (06-20-2023)

Our lab has been using Proteintech's beta actin polyclonal antibody for years. We use this antibody in all our western blot experiments for normalization. This antibody works consistently at a 1:1000 dilution for human and mouse retina and for HRECs. We have always gotten a beautiful result, with nice thick and specific bands. The only downside is that this antibody is hard to strip. Even after stripping, the membrane tends to have residual beta actin bands. Even so, we would highly recommend this product for routine western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse retina, human retina, human retinal endothelial cell line

Beta Actin Antibody Western Blot validation (1:1000 dilution) in mouse retina, human retina, human retinal endothelial cell line (Cat no:20536-1-AP)

FH

Chun (Verified Customer) (06-19-2022)

This antibody worked very well.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HeLa cells and HEK293 cells

FH

Aaron (Verified Customer) (12-08-2020)

nice bands achieved

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Rat epidermal keratinocytes

FH

Wei (Verified Customer) (01-30-2020)

Strong, clear band for basal heart tissues.Good for internal control for heart diseases.

• Applications: Western Blot,
• Cell Tissue Type: Mouse Heart

FH

SCOTT (Verified Customer) (10-11-2019)

Used 1/1000 overnight 4 C. overnightHuman Grey matter cortex post mortem material

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human Grey matter cortex post mortem

Beta Actin Antibody Western Blot, validation (1/1000 dilution) in Human Grey matter cortex post mortem (Cat no:20536-1-AP)

FH

Dan (Verified Customer) (08-19-2019)

The antibody works

• Applications: Western Blot, Immunohistochemistry,

FH

Kishor (Verified Customer) (01-30-2019)

Working well for Western Blotting (1:5000)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: H69, HUCCT-1 and Rat Liver tissue

Beta Actin Antibody Western Blot, validation (1:5000 dilution) in H69, HUCCT-1 and Rat Liver tissue (Cat no:20536-1-AP)

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