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BAIAP2L1 Polyklonaler Antikörper

BAIAP2L1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25692-1-AP

Synonyme

BAIAP2L1, IRTKS

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of HeLa cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of HeLa using 25692-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of K-562 using 25692-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    WB analysis of SGC-7901 using 25692-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human ovary tumor using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using BAIAP2L1 Polyclonal antibody (25692-1-AP)

    IHC staining of human stomach cancer using 25692-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25692-1-AP (BAIAP2L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, K-562-Zellen, SGC-7901-Zellen
Erfolgreiche Detektion in IHChumanes Ovarialkarzinomgewebe, humanes Magenkrebsgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25692-1-AP bindet in WB, IHC,ELISA BAIAP2L1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen BAIAP2L1 fusion protein Ag22499
Vollständiger Name BAI1-associated protein 2-like 1
Berechnetes Molekulargewicht 511 aa, 57 kDa
Beobachtetes Molekulargewicht 57 kDa
GenBank-ZugangsnummerBC013888
Gene symbol BAIAP2L1
Gene ID (NCBI) 55971
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAIAP2L1, also known as IRTKS, is a 511-amino acid protein containing an IRSp53/MIM homology domain (IMD) that bundles actin filaments and interacts with the small GTPase Rac (PMID: 17430976; 14752106). In addition to the IMD domain, BAIAP2L1 also contains an SH3 domain and WW domain interaction motif. BAIAP2L1 is widely distributed and is an INS receptor substrate. It has a role in actin remodeling and membrane protrusion.

Protokolle

Produktspezifische Protokolle
WB protocol for BAIAP2L1 antibody 25692-1-APProtokoll herunterladen
IHC protocol for BAIAP2L1 antibody 25692-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

anwesh (Verified Customer) (04-01-2019)

Great antibody. Detects both human and mouse versions of the protein.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: 293T and overexpression
BAIAP2L1 Antibody Western Blot, validation (1:1000 dilution) in 293T and overexpression (Cat no:25692-1-AP)